Quantitative proteomic analyses reveal that energy metabolism and protein biosynthesis reinitiation are responsible for the initiation of bolting induced by high temperature in lettuce (Lactuca sativa L.)
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ABSTRACT: Lettuce (Lactuca sativa L.) as one of the most economically important leaf vegetables, is easy bolting in high temperature condition. Early bolting loses its commodity value and edible value and bring huge losses and waste of resources. However, the initiation and molecular mechanism underlying early bolting induced by high temperature remains largely elusive. To better understand it, a comparative proteomics analysis was conducted on lettuce, in the initiation period of bolting induced by a high temperature (33 oC) and a control temperature (20 oC) using iTRAQ-based proteomics, phenotypic measures, and biological verifications using qRT-PCR. The morphologic and microscopic observation showed that the initiation of bolting was on 8 day after high temperature treatment. Fructose rapidly accumulated after high temperature. In the period of the bolting initiation, of the 3305 identified proteins, a total of 93 proteins were identified with differential abundance, among 38 of these proteins were up-regulated and 55 proteins were down-regulated. About 38% of proteins were involved in metabolic pathway and mainly clustered in energy metabolism and protein synthesis. Furthermore, some proteins involved in sugar synthesis were differentially expressed, which were also related with energy production. This is the first report on the metabolic changes involved in bolting initiation in lettuce. Our study suggested that energy metabolism and ribosomal proteins are pivotal components during bolting initiation. It may bring potential regulatory mechanism for the initiation of early bolting by high temperature and manipulations for breeding in lettuce.
ORGANISM(S): Lactuca Sativa Var. Capitata
SUBMITTER: Jinghong Hao
PROVIDER: PXD014464 | iProX | Tue Jul 02 00:00:00 BST 2019
REPOSITORIES: iProX
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