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Integrative proteomics of macrophage in response to LPS stimulation

ABSTRACT: PTM proteomics had been increasingly used in studies related to inflammation and immunity, but the integrated analysis of multiple PTM was lacking. Here, we integrated WCP, acetylome, phosphoproteome and ubiquitinome datasets to identify LPS stimulation-dependent PTM events in macrophages with or without MG132. Using the SILAC quantification, we successfully identified 53,785 PTM sites and 19,726 proteins at all times in LPS-stimulated macrophages. Integration of these datasets provided a possibility to have a more comprehensive understanding of the inflammatory response.

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Lanjuan Li

PROVIDER: PXD015527 | iProX | Fri Sep 20 00:00:00 BST 2019

REPOSITORIES: iProX

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Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response.

Ji Feiyang F Zhou Menghao M Zhu Huihui H Jiang Zhengyi Z Li Qirui Q Ouyang Xiaoxi X Lv Yiming Y Zhang Sainan S Wu Tian T Li Lanjuan L

Genomics, proteomics & bioinformatics 20210302 1

Posttranslational modifications (PTMs) of proteins, particularly acetylation, phosphorylation, and ubiquitination, play critical roles in the host innate immune response. PTMs' dynamic changes and the crosstalk among them are complicated. To build a comprehensive dynamic network of inflammation-related proteins, we integrated data from the whole-cell proteome (WCP), acetylome, phosphoproteome, and ubiquitinome of human and mouse macrophages. Our datasets of acetylation, phosphorylation, and ubiq ...[more]

PMID: 33662623

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