Dataset Information

Time-resolved extracellular matrix atlas of the developing human skin dermis

ABSTRACT: Skin aging is a physiological problem that remains a concern. Studies have demonstrated that the dermal extracellular matrix (ECM) plays important roles in skin aging; however, changes in ECM characteristics and molecules that are secreted to the extracellular space and involved in the formation of dermal matrix from birth to aging remain unclear. Furthermore, it is not understood how the ECM microenvironment supports the functions of skin development across different age groups. We used a decellularization method and matrisome analysis to compare the composition, expression, and function of the dermal ECM in neonate, teenage, adult, and elderly skin. We found that the collagens, glycoproteins, proteoglycans, and regulatory factors that support skin development and function together with these core ECM proteins were differentially expressed at different ages. We also identified ECM expression markers during the process of skin development. Our results systematically revealed the characteristics of ECM synthesis, response to growth factors, water conservation, anti-oxidation, and the ability of the ECM to support epidermal stem cell growth via the basement membrane in young skin, as well as the characteristics of response to wound repair and fungal invasion in aging skin.

ORGANISM(S): Homo Sapiens

SUBMITTER: Ling Leng

PROVIDER: PXD016440 | iProX | Mon Nov 28 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications

Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.

Li Mansheng M Li Xiao X Liu Binghui B Lv Luye L Wang Wenjuan W Gao Dunqin D Zhang Qiyu Q Jiang Junyi J Chai Mi M Yun Zhimin Z Tan Yingxia Y Gong Feng F Wu Zhihong Z Zhu Yunping Y Ma Jie J Leng Ling L

Frontiers in cell and developmental biology 20211126

Skin aging is a physiological issue that is still relatively poorly understood. Studies have demonstrated that the dermal extracellular matrix (ECM) plays important roles in skin aging. However, the roles of the changes in ECM characteristics and the molecules that are secreted to the extracellular space and are involved in the formation of the dermal matrix from birth to old age remain unclear. To explore the way in which the ECM microenvironment supports the functions of skin development acros ...[more]

PMID: 34901026

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Project description:Col1a1 is located on mouse distal chromosome 11 (Celera 94607393-94622990bp; 15kb) and encodes type I procollagen that associates stoichiometrically with col1a2 in forming secreted type I mature collagen that self-assembles into a supramolecular fibrillar structure consisting of a protein family. Type I collagens are predominantly enriched in bone, cartilage, skin, tendons, and eye (i.e. in major fibrous tissues) making up the main extracellular matrix (ECM) component for support and scaffolding purposes. Col1a1 is known to be expressed in the embryo (E9.5; Northern dot blot), perioptic mesenchyme (E11), cornea (E11), skeleton (E14.5), brain meninges (E14.5), and cartilage (E14.5) at relatively early stages. Most evidence of type I collagen function is coupled to the latter stages of skeletal development, thus bypassing the major events of axis formation, tissue differentiation, and mesenchlymal-epithial interactions, for example, that are more symbolic of morphogens and growth factors. Members of the integrin cell surface receptor family of molecules mediate cell adhesion to ECM proteins such as collagen (mainly to type IV basement membrane collagen that forms into a polygonal meshwork), fibronectin, and laminin implicated in wound healing and inflammatory responses (e.g. Crohn disease, ulcerative colitis) in connective tissues. There is some evidence for protein-protein interactions between leukocyte-associated integrins and the interstitial matrix in promoting the migration and/or activation of extravasated leukocytes (e.g., T cells and monocytes) within the perivascular compartment, an ECM-rich environment. This region is surmised to be an important location where certain human leukocytes undergo differentiation (e.g. monocytes) and activation (neutrophils, monocytes, lymphocytes) upon extravascular migration. The von Willebrand factor (vWF), type C motif is found in various plasma proteins like complement factors, the integrins, collagen types, and other extracellular proteins. Thus, the majority of vWF-containing proteins are extracellular and a common feature appears to be involvement in multi protein complexes participating in numerous biological events (e.g. cell adhesion, migration, homing, pattern formation, and signal transduction). Three organs (lung, heart and bone) of 5 AGA002 mutant mice (age 2x 10 days and 3x 11 days) were analysed by cDNA microarray technology. As reference five mice, 11 days old were used 50% of the chip hybridisations are dye swap experiments.

Dataset Information

Time-resolved extracellular matrix atlas of the developing human skin dermis

Publications

Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.

Similar Datasets

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