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LysargiNase and Chemical Derivatization Based Strategy for 2 Facilitating In-Depth Profiling of C‑Terminome

ABSTRACT: we developed a new method for C-terminomics, in which LysargiNase and chemical derivatization were integrated to increase the identification confidence.

ORGANISM(S): Homo Sapiens

SUBMITTER: Minjia Tan

PROVIDER: PXD016756 | iProX | Mon Dec 16 00:00:00 GMT 2019

REPOSITORIES: iProX

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LysargiNase and Chemical Derivatization Based Strategy for Facilitating In-Depth Profiling of C-Terminome.

Hu Hao H Zhao Wensi W Zhu Mengdi M Zhao Lei L Zhai Linhui L Xu Jun-Yu JY Liu Ping P Tan Minjia M

Analytical chemistry 20191105 22

Global identification of protein C-termini is highly challenging due to their low abundance in conventional shotgun proteomics. Several enrichment strategies have been developed to facilitate the detection of C-terminal peptides. One major issue of previous approaches is the limited C-terminome coverage. Herein, we integrated LysargiNase digestion, chemical acetylation on neo-N-terminus, and a-ion-aided peptide matching into poly(allylamine)-based C-terminomics (termed as LAACTer). In this strat ...[more]

PMID: 31634432

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