Dataset Information

Chloroplast proteomics of wheat under drought

ABSTRACT: Perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. Exogenous application of 5-aminolevulinic acid (ALA) mitigates the damages caused by drought stress protecting plant growth and development, but the regulatory mechanism behind was still obscure. Wheat seedlings were drought-treated, and the iTRAQ based proteomics approach was employed to assess the difference in chloroplast protein expression regulation caused by exogenous ALA. A total of 9499 peptides were identified with ≤ 0.01 FDR, which could be classified into 2442 protein groups. Moreover, the expressions of 87 chloroplast proteins were changed by drought stress alone compared to drought-free control, while 469 were changed by exogenous ALA application under drought stress compared to drought stress alone. The Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results suggested that ALA pre-treatment adjusted some biological pathways to enhancing the drought resistance of chloroplast, such as metabolic pathways, photosynthesis, and ribosome. Furthermore, the drought promoted H2O2 accumulation and O2- production in chloroplast were alleviated by exogenous pre-treatment of ALA, while the peroxidase (POD) and glutathione peroxidase (GPX) activities were up-regulated, which were coincident with the chloroplast proteomics data. We suggested that ALA promoted the reactive oxygen species (ROS) scavenging in chloroplast by regulating enzymatic processes. Our results extend the understanding of the mechanisms employed by exogenous ALA to defend against drought stress in wheat according to the chloroplast proteomics.

ORGANISM(S): Triticum Aestivum

SUBMITTER: Yuexia Wang

PROVIDER: PXD017528 | iProX | Mon Dec 10 00:00:00 GMT 2018

REPOSITORIES: iProX

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Publications

The iTRAQ-based chloroplast proteomic analysis of Triticum aestivum L. leaves subjected to drought stress and 5-aminolevulinic acid alleviation reveals several proteins involved in the protection of photosynthesis.

Wang Yuexia Y Li Xiaoyan X Liu Nana N Wei Shimei S Wang Jianan J Qin Fujun F Suo Biao B

BMC plant biology 20200304 1

<h4>Backgrounds</h4>The perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. The exogenous application of 5-aminolevulinic acid (ALA) mitigates the damage caused by drought stress, protecting plant growth and development, but the regulatory mechanism behind this process remains obscure.<h4>Results</h4>Wheat seedlings were drought treated, and the iTRAQ-based proteomic approach was employed to assess the difference in chloroplast protein content ...[more]

PMID: 32131734

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Project description:In the field, abiotic stresses are rarely applied individually. Crops are often subjected to a combination of stresses. To date, no study has been performed on the proteomic investigation of the response of common wheat to a combination of drought and cold stresses. In this study, wheat seedlings exposed to drought-cold stress for 24 h showed inhibited growth, increased lipid peroxidation, relative electrolyte leakage, and soluble sugar contents. To determine the wheat protein response to drought-cold stress, iTRAQ-based quantitative proteomic and liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were employed to determine the proteomic profiles of the roots and leaves of wheat seedlings exposed to drought-cold stress conditions. We identified 250 and 258 proteins with significantly altered abundance in the roots and leaves, respectively. These proteins were classified into several main groups, as follows: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, transcription-related processes, energy production, cell-wall and cytoskeleton metabolism, membrane and transportation, signal transduction, other metabolic processes, and unknown biological processes. Nine proteins were simultaneously presented in both roots and leaves exposed to drought-cold stress, and the majority of proteins identified differed from one another and displayed differently altered abundance. These findings uncovered organ-specific differences in adaptation to drought-cold stress. Exogenous abscisic acid (ABA) application conferred the plant with protection against drought-cold stress and significantly increased catalase and peroxidase enzyme activities, as well as the transcription of glutathione S-transferase and other 11 sample genes in the roots or leaves, respectively. These results suggested that ABA is a potentially vital factor that contributes to the drought-cold signaling pathway and a promising target for growth recovery. Furthermore, VIGS (virus-induced gene silencing)-treated plants generated for three candidate protein genes TaGRP2, CDCP and WCOR410c were subjected to drought-cold limitation, they showed more serious droop and wilt, increased rate of relative electrolyte leakage, and reduced relative water content (RWC) compared to viral control plants. These results may indicate that TaGRP2, CDCP and WCOR410c play important roles in conferring drought-cold tolerance in wheat. These findings can provide useful insights into the molecular mechanisms of drought-cold responses in higher plants.

Dataset Information

Chloroplast proteomics of wheat under drought

Publications

The iTRAQ-based chloroplast proteomic analysis of Triticum aestivum L. leaves subjected to drought stress and 5-aminolevulinic acid alleviation reveals several proteins involved in the protection of photosynthesis.

Similar Datasets

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