Proteomics

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Proteomic analysis of soybean root including hypocotyl during recovery after drought stress


ABSTRACT: Soybean is a nutritionally important crop that exhibits reductions in growth and yields under drought stress. To investigate soybean responses during post-drought recovery, a gel-free proteomic technique was used to examine the protein profile. Two-day-old soybeans were stressed with drought for 4 days, recovered for 4 days, and root including hypocotyl was collected under drought and during the recovery stage. Morphological analysis revealed that growth was suppressed under drought stress that recovered following stress removal. Malondialdehyde content was increased under drought stress but returned to normal during the recovery stage. A total of 792 and 888 proteins were identified in control and drought-stressed root including hypocotyl during recovery stage, respectively. Based on the proteomic and cluster analyses, peroxidase and aldehyde dehydrogenase were significantly changed at analyzed time points under drought stress and during recovery. The activity of peroxidase was decreased under drought stress but increased during recovery. The activity of aldehyde dehydrogenase was increased under drought stress but returned to normal during the recovery stage. These results suggest that peroxidase and aldehyde dehydrogenase play key role in post-drought recovery in soybean by scavenging toxic reactive oxygen species and reducing harmful aldehydes load.

INSTRUMENT(S): LTQ XL

ORGANISM(S): Glycine max  

TISSUE(S): Root

DISEASE(S): Wounds And Injuries

SUBMITTER: Mudassar Khan  

LAB HEAD: Setsuko KOMATSU

PROVIDER: PXD003650 | Pride | 2016-06-16

REPOSITORIES: pride

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Publications

Proteomic analysis of soybean root including hypocotyl during recovery from drought stress.

Khan Mudassar Nawaz MN   Komatsu Setsuko S  

Journal of proteomics 20160610


Soybean is a nutritionally important crop that exhibits reductions in growth and yield under drought stress. To investigate soybean responses during post-drought recovery, a gel-free proteomic technique was used. Two-day-old soybeans were treated with drought stress for 4days and recovered for 4days. Root including hypocotyl was collected during the drought treatment and recovery stage. Seedling growth was suppressed by drought stress, but recovered following stress removal. The malondialdehyde  ...[more]

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