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The quantitative and phosphorylation proteomics analysis of mouse liver after tick-borne Babesia microti infection

ABSTRACT: Babesia microti is a tick-borne protozoan parasite that infects the red blood cells of mice, humans, and other mammals. Hosts infected with B. microti exhibit a series of clinical symptoms, including fever, anaemia, and haemoglobinuria, and multiple-organ injury. The infection can even result in death in patients with severe symptoms. Here, after BALB/c mice were infected with B. microti, liver tissues had serious injury and obvious swelling, cells were deformed, lipid droplets had formed in the cytoplasm, and organelles such as mitochondria also exhibited morphological changes. To further investigate the molecular mechanisms underlying liver injury and liver self-repair after B. microti infection, data-independent acquisition quantitative (DIA) proteomics was used to analyse changes in the expression and phosphorylation of proteins in liver tissues in the infection period and recovery period after BALB/c mice were infected with B. microti. The pattern of changes and the functions of proteins in lipid metabolism, growth and development, and immunity during liver injury and repair were investigated.

ORGANISM(S): Mus Musculus

SUBMITTER: Hui Wang

PROVIDER: PXD018102 | iProX | Thu Mar 19 00:00:00 GMT 2020

REPOSITORIES: iProX

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Quantitative proteomics and phosphoproteomic analyses of mouse livers after tick-borne Babesia microti infection.

Hu Yuhong Y Wang Minjing M Ren Shuguang S Masoudi Abolfazl A Xue Xiaomin X Wang Xiaoshuang X Yang Xiaohong X Han Yanan Y Li Mengxue M Wang Hui H Liu Jingze J

International journal for parasitology 20201123 2-3

Babesia microti is a tick-borne protozoan parasite that infects the red blood cells of mice, humans, and other mammals. The liver tissues of BALB/c mice infected with B. microti exhibit severe injury. To further investigate the molecular mechanisms underlying liver injury and liver self-repair after B. microti infection, data-independent acquisition (DIA) quantitative proteomics was used to analyse changes in the expression and phosphorylation of proteins in liver tissues of BALB/c mice during a ...[more]

PMID: 33242464

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Project description:Background: Fasciola hepatica infection still remains one of the helminthic neglected tropical diseases (NTDs). It has a huge worldwide distribution, affecting mainly cattle and, sometimes, human beings. In addition to data reported about the immunological response induced by helminthic infections and that induced by Fasciola hepatica, little is known about the gene expression profile in its organ target, the liver, which is where adult worms are established and live for long periods of time, causing its characteristic pathology. In the present work, we study both the early and late gene expression profiles in the livers of mice infected with Fasciola hepatica metacercariae using a microarray-based methodology. Methodology: A total of 9 female-6-week-old BALB/c mice (Charles River Laboratories, Barcelona, Spain) weighing 20 to 35 g were used for the experiments. Two groups of BALB/c mice were orally infected with seven F. hepatica metacercariae, and the other group remained untreated and served as a control. Mice were humanely euthanized and necropsied for liver recovery, histological assessment of hepatic damage, RNA isolation, microarray design and gene expression analysis on the day of infection (t0), seven days post-infection (t7) and twenty-one days post-infection (t21). Results: We found that Fasciola hepatica infection induces the differential expression of 128 genes in the liver in the early stage of infection and 308 genes in the late stage, and most of them are up-regulated. The Ingenuity Pathway Analysis revealed significant changes in the pathways related to metabolism, biosynthesis and signaling as well as genes implicated in inducing liver-toxicity, injury and death. Conclusion: The present study provides us insights at the molecular level about the underlying mechanisms used by Fasciola hepatica, leading to liver damage and its subsequent pathophysiology. The expression pattern obtained here could also be used to explain the lack of association between infection with F. hepatica and cholangiocarcinoma. However, more studies should be performed to confirm this hypothesis. We used three experimental groups each containing 3 mice. Group 1 remains untreated and served as control. Group 2 was infected with Fasciola hepatica metacercariae on day 0 and humanely necropsied at 7 days post-infection. Group 3 was infected with Fasciola hepatica metacercariae on day 0 and humanely necropsied at 21 days post- infection. At the time of necropsy, liver of each mice were removed and the RNA was isolated. We compared the gene expression profile in the liver of mice infected with Fasciola hepatica.

Dataset Information

The quantitative and phosphorylation proteomics analysis of mouse liver after tick-borne Babesia microti infection

Publications

Quantitative proteomics and phosphoproteomic analyses of mouse livers after tick-borne Babesia microti infection.

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