Proteomics

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The proteomic changes in Pseudobagrus vachelli muscle and brain induced by hypoxia

ABSTRACT: The present NGST, TMT and Q-TOF MS platform should provide unprecedented resources to address such questions as to how hypoxic condition affects gene, miRNA, protein, and metabolite expression and changes the molecular pathways, and whether miRNAs participate in this process. For this purpose, we characterise transcriptomic, miRNAomic, proteomic and metabonomic sequencing of control- and hypoxia-treated P. vachelli muscles to elucidate the molecular mechanisms of hypoxia adaptation. We were able to find the predicted miRNA-mRNA-protein-metabolite regulatory network using bioinformatics analysis and miRNA prediction algorithms (Fig. 1). This is the first report on integrated analysis of transcriptome, miRNAome, and proteome, and metabolome in fishes and as such offers deeper insight into the hypoxia molecular mechanisms. We provide a good case study with which to analyse mRNA, miRNA, protein and metabolite expression and profile non-model fish species.

ORGANISM(S): Tachysurus Vachellii

SUBMITTER: Shaowu Yin  

PROVIDER: PXD020425 | iProX | Sat Jul 18 00:00:00 BST 2020

REPOSITORIES: iProX

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Integrated Application of Multiomics Strategies Provides Insights Into the Environmental Hypoxia Response in Pelteobagrus vachelli Muscle.

Li Jie J   Zhang Guosong G   Yin Danqing D   Li Yao Y   Zhang Yiran Y   Cheng Jinghao J   Zhang Kai K   Ji Jie J   Wang Tao T   Jia Yongyi Y   Yin Shaowu S  

Molecular & cellular proteomics : MCP 20220111 3

Increasing pressures on aquatic ecosystems because of pollutants, nutrient enrichment, and global warming have severely depleted oxygen concentrations. This sudden and significant lack of oxygen has resulted in persistent increases in fish mortality rates. Revealing the molecular mechanism of fish hypoxia adaptation will help researchers to find markers for hypoxia induced by environmental stress. Here, we used a multiomics approach to identify several hypoxia-associated miRNAs, mRNAs, proteins,  ...[more]

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