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Phosphoproteome of honeybee brain

ABSTRACT: A. meliffera and A. cerana are western and eastern honeybee species that evolve a wide spectrum of differences in periphery olfactory system and olfactory driven behaviors. However, the knowledge about the central nervous system regulating the olfaction differences between two bee species is largely unknown. Here, an in-depth phosphoproteome of the antennal lobes (ALs) between two bee species was comparied. Totally, 1227 phosphoprotein, 2951 phosphopeptide and 3906 phosphosite were identified, of which 76% phosphoproteins related to cytoskeleton formation and signal transduction were shared between both bee species, indicates that protein phosphorylation plays a fundamental role in ALs glomeruli formation, synaptic plasticity and signal transduction. Furthermore, 15% (191) and 8.4% (107) phosphoproteins were uniquely expressed in ALs of A. mellifera and A. cerana, respectively, they were mainly involved in ion transmembrane transport, affects ion channel activity. Addtionally, mTOR signaling pathway, a central player of protein synthesis and synaptic plasticity, were enriched in ALs, suggests its important role in olfactory processing, learning and memory formation. Notably, the key phosphoproteins of mTOR pathway were up-regulated in A. cerana ALs relative to A. meliffera, suggests that mTOR signaling pathway may driven the difference olfactory learning and memory ability between two bee species via modification of synaptic plasticity. This data provide firstly comprehensive phosphoproteome profile of both honeybee ALs, and it is potentially useful for further analysis of the neuronal mechanism for the olfactory process by phosphorylation.

ORGANISM(S): Apis Mellifera

SUBMITTER: Jianke Li

PROVIDER: PXD022900 | iProX | Thu Dec 03 00:00:00 GMT 2020

REPOSITORIES: iProX

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Project description:Honeybee brain has distHoneybee brain has distinct anatomical and functional regions, knowledge on molecular underpinnings of sub-organ to achieve the distinct neural function and the difference between the eastern and western honeybees are still missing. Here, the proteomes of three sub-organs of eastern and western honeybee brains were compared. Mushroom bodies (MBs) and optical lobes (OLs) may by employed similar proteome architectures to drive their domain-specific neural activity in both bee species. In MBs, protein metabolism and Ca2+ transmembrane transport are the key role players in driving the learning and memory by modulating the synaptic structure and signal transduction to consolidate memory trace. In OLs, ribonucleoside metabolism and energy production play major roles to underpin visual system by maintaining G-protein cycle and membrane electrical charge potential. However, in antennal lobes (ALs), it has evolved distinct proteome settings to prime the olfactory learning and memory in two bee species. In ALs of Apis cerana cerana (Acc), actin cytoskeleton organization is key for plasticity of glomeruli and intracellular transport to sustain the olfactory signaling. Whereas, in ALs of Apis mellifera ligustica (Aml), hydrogen and hydrogen ion transport are vital to support olfactory process by supplying energy and maintaining molecule transport. Noticeably, in ALs of Acc, the exclusively enriched functional groups acting as second messenger and neurontransmitter of signal transduction, and the enhanced protein metabolism to regulate the plasticity of synaptic structure for formation of memory, suggest that Acc may have evolved a better sense of smell than that of Aml. Our first proteome data is helpful as starting point for further analysis of neural activity in brain sub-area of honeybee and other insects.inct anatomical and functional regions, knowledge on molecular underpinnings of sub-organ to achieve the distinct neural function and the difference between the eastern and western honeybees are still missing. Here, the proteomes of three sub-organs of eastern and western honeybee brains were compared. Mushroom bodies (MBs) and optical lobes (OLs) may by employed similar proteome architectures to drive their domain-specific neural activity in both bee species. In MBs, protein metabolism and Ca2+ transmembrane transport are the key role players in driving the learning and memory by modulating the synaptic structure and signal transduction to consolidate memory trace. In OLs, ribonucleoside metabolism and energy production play major roles to underpin visual system by maintaining G-protein cycle and membrane electrical charge potential. However, in antennal lobes (ALs), it has evolved distinct proteome settings to prime the olfactory learning and memory in two bee species. In ALs of Apis cerana cerana (Acc), actin cytoskeleton organization is key for plasticity of glomeruli and intracellular transport to sustain the olfactory signaling. Whereas, in ALs of Apis mellifera ligustica (Aml), hydrogen and hydrogen ion transport are vital to support olfactory process by supplying energy and maintaining molecule transport. Noticeably, in ALs of Acc, the exclusively enriched functional groups acting as second messenger and neurontransmitter of signal transduction, and the enhanced protein metabolism to regulate the plasticity of synaptic structure for formation of memory, suggest that Acc may have evolved a better sense of smell than that of Aml. Our first proteome data is helpful as starting point for further analysis of neural activity in brain sub-area of honeybee and other insects.

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Phosphoproteome of honeybee brain

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