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MS characterization of SARS-CoV-2 NCP PTM profile

ABSTRACT: Glycosylation and PTM profile of NCP from SARS-CoV-2

ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2

SUBMITTER: Zeyu Sun

PROVIDER: PXD025404 | iProX | Wed Apr 14 00:00:00 BST 2021

REPOSITORIES: iProX

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Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.

Sun Zeyu Z Ren Keyi K Zhang Xing X Chen Jinghua J Jiang Zhengyi Z Jiang Jing J Ji Feiyang F Ouyang Xiaoxi X Li Lanjuan L

Engineering (Beijing, China) 20200830 10

The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus. In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by <i>N</i>-glycans, predominantly of the oligomannose type. All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely ...[more]

PMID: 32904601

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