Phosphorylation events during systemic acquired resistance in Arabidopsis
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis Thaliana
SUBMITTER: 
Ziqin Xu
PROVIDER: PXD027512 | iProX | Fri Jul 23 00:00:00 BST 2021
REPOSITORIES: iProX
Publications
Protein Phosphorylation Changes During Systemic Acquired Resistance in <i>Arabidopsis thaliana</i>.
Zhou Qingfeng Q Meng Qi Q Tan Xiaomin X Ding Wei W Ma Kang K Xu Ziqin Z Huang Xuan X Gao Hang H
Frontiers in plant science 20211111
Systemic acquired resistance (SAR) in plants is a defense response that provides resistance against a wide range of pathogens at the whole-plant level following primary infection. Although the molecular mechanisms of SAR have been extensively studied in recent years, the role of phosphorylation that occurs in systemic leaves of SAR-induced plants is poorly understood. We used a data-independent acquisition (DIA) phosphoproteomics platform based on high-resolution mass spectrometry in an <i>Arabi ...[more]
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Project description:<p><strong>BACKGROUND:</strong> Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen resistance to uninfected distal leaves following an initial localized infection. However, little information is available on the molecular biological basis of SAR especially in uninfected distal leaves. </p><p><strong>METHODS:</strong> Here, we used two SAR-inducing pathogen to induce SAR in Arabidopsis, and a metabolomics approach based on ultra-high-performance liquid chromatography (UPLC) and mass spectrometry (MS) technique was used to identify SAR-related metabolites in SAR-inducing pathogen infected local leaves and uninfected distal leaves. </p><p><strong>RESULTS:</strong> Multiple statistical analyses were used to identify differentially expressed metabolites. The result showed that primary metabolism and secondary metabolism were significantly altered in local leaves and distal leaves, including phenolic compounds, amino acids, nucleotides, organic acids and many other metabolites.</p><p><strong>CONCLUSIONS:</strong> The content of amino acids and phenolic compounds increased in distal leaves, suggesting their contribution to the establishment of SAR in distal leaves. In addition, 2’-hydroxy-4, 4’, 6’-trimethoxychalcone, phenylalanine and p-coumaric acid were identified as potential components which may play important roles both in basic resistance and SAR. This study provided a reference for understanding metabolic mechanism associated with SAR in Arabidopsis, which will be useful for further investigation of the molecular basis of SAR.</p>
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