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Proteomic and phosphoproteomic landscape reveals the functional contributions of circadian gene Bmal1 in rat nucleus pulposus cells

ABSTRACT: Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), and the pathogenesis remains unknown. Recently, an autoregulating circadian rhythm was identiﬁed in intervertebral discs (IVDs), the abolition of which led to IDD. The genetic disruption of the mouse IVD molecular clock, speciﬁcally through the disruption of Bmal1, predisposes mice to IDD. However, the precise role of circadian gene Bmal1 in IDD remain elusive. Using a tandem mass tag (TMT)-based quantitative proteomics approach, we characterized the proteomes and phosphoproteomes of rat nucleus pulposus cells (NPCs) treated with Bmal1 shRNA or its negative control lentivirus.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Jinghui Huang

PROVIDER: PXD029273 | iProX | Thu Oct 21 00:00:00 BST 2021

REPOSITORIES: iProX

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Hsa-let-7f-1-3p targeting the circadian gene Bmal1 mediates intervertebral disc degeneration by regulating autophagy.

Mei Liangwei L Zheng Yi Y Gao Xue X Ma Teng T Xia Bing B Hao Yiming Y Wei Bin B Wei Yitao Y Luo Zhuojing Z Huang Jinghui J

Pharmacological research 20221102

The intervertebral disc has an intrinsic circadian rhythm, elimination of which leads to stress in nucleus pulposus cells (NPCs), contributing to intervertebral disc degeneration (IDD). Disruption or deletion of Bmal1 (a core transcription factor) results in complete loss of circadian rhythms, make mice susceptibility to IDD. However, the underlying mechanism by which Bmal1 mediates IDD is remains enigmatic, and whether there are other upstream factors regulating Bmal1 in NPCs. In our study, we ...[more]

PMID: 36334875

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