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SCN Proteomic Study:electroacupuncture induced circadian control in Suprachiasmatic Nucleus

ABSTRACT: Recent reports highlight phosphorylation as a major role in the regulation of sleep-wake cycles, to a lesser extent, the phosphoproteome in Suprachiasmatic Nucleus (SCN) is not well understood. We use multiplex tandem mass tag (TMT) - labelling coupled with liquid chromatography-mass spectrometry (LC-MS) to study the rhythmic regulatory effects of electroacupuncture(EA) at different times in constant darkness and the mechanism of EA mediated SCN protein phosphorylation. We applied the mass spectrometry-based phosphoproteome to explore the molecular characteristics of EA-induced phosphorylation modification in the SCN.

ORGANISM(S): Mus Musculus

SUBMITTER: Dingjun Cai

PROVIDER: PXD029450 | iProX | Sun Oct 31 00:00:00 BST 2021

REPOSITORIES: iProX

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Synaptic Protein Phosphorylation Networks Are Associated With Electroacupuncture-Induced Circadian Control in the Suprachiasmatic Nucleus.

Lu Xiaoxiao X Zhou Minjie M Liu Nannan N Zhang Chengshun C Zhao Zhengyu Z Cai Dingjun D

Frontiers in genetics 20211216

Phosphorylation is one of the most important posttranslational modifications and regulates the physiological process. While recent studies highlight a major role of phosphorylation in the regulation of sleep-wake cycles to a lesser extent, the phosphoproteome in the suprachiasmatic nucleus (SCN) is not well-understood. Herein, we reported that the EA treatment elicits partial reparation of circadian rhythmicity when mice were exposure to constant darkness for long time. We investigated the effec ...[more]

PMID: 34976011

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