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Comparative maternal protein profiling of mouse biparental and uniparental embryos

ABSTRACT: During oogenesis, RNAs and proteins from maternal genome will be accumulated and they are the most important regulatory factors in early embryonic development. Parthenogenesis provides a unique source to investigate the influence of maternal genomes in early mammalian development and is proposed as an experimental tool to investigate embryo development which may solve many of the ethical concerns. Using label-free quantitative mass sepctrometry (MS), we systematically monitored protein expression profiles from six stages during pre-implantation development used by parthenogenesis model of mouse: pronucleus-, 2-cell, 4-cell, and 8-cell embryo, morula, and blastocyst. They are labeled as PA, PA-2, PA-4, PA-8, PAMO, PABL, respectively. For each stage, 6,000 embryos were used, and the experiment was performed in three biological replicates, and they had 2,048 proteins in common.

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Fumei Chen

PROVIDER: PXD029532 | iProX | Fri Jul 15 00:00:00 BST 2022

REPOSITORIES: iProX

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Comparative maternal protein profiling of mouse biparental and uniparental embryos.

Chen Fumei F Ma Buguo B Lin Yongda Y Luo Xin X Xu Tao T Zhang Yuan Y Chen Fang F Li Yanfei Y Zhang Yaoyao Y Luo Bin B Zhang Qingmei Q Xie Xiaoxun X

GigaScience 20220901

<h4>Background</h4>Maternal proteins have important roles during early embryonic development. However, our understanding of maternal proteins is still very limited. The integrated analysis of mouse uniparental (parthenogenetic) and biparental (fertilized) embryos at the protein level creates a protein expression landscape that can be used to explore preimplantation mouse development.<h4>Results</h4>Using label-free quantitative mass spectrometry (MS) analysis, we report on the maternal proteome ...[more]

PMID: 36056732

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