Dataset Information

Toxicity of chlorpyrifos to the ovary of Haemaphysalis longicornis and its effect on the expression of ovarian protein

ABSTRACT: A tick is an ectoparasitic arthropod that sucks blood exclusively. It is widely distributed all over the world. Haemaphysalis longicornis has strong fecundity, and each female tick can lay thousands of eggs. The ovary is an important part of the reproductive system of female ticks and plays an important role in the reproductive process of female ticks. Therefore, the research on tick ovaries is of great significance for the effective prevention and control of this species. In this study, data-independent acquisition (DIA) proteomics was used to study the ovary of H. longicornis treated with organophosphorus insecticide chlorpyrifos. The dynamic changes of protein in the ovary of H. longicornis were analyzed under different doses of chlorpyrifos and different treatment time. The results showed that these differentially expressed proteins mainly included proteins related to material metabolism and energy regulation. They are mainly involved in the metabolic pathway, TCA cycle pathway, glycolysis and glycogenesis pathway, peroxisome pathway, and so on. This study not only further explores the molecular mechanism of tick tolerance to chlorpyrifos, but also provides new molecular targets and ideas for the development of new anti-tick vaccines and anti-tick drugs, which is of great significance in the integrated control of ticks.

ORGANISM(S): Haemaphysalis Longicornis

SUBMITTER: Hui Wang

PROVIDER: PXD029923 | iProX | Fri Nov 26 00:00:00 GMT 2021

REPOSITORIES: iProX

ACCESS DATA

Similar Datasets

Project description:Tick saliva contains many bioactive molecules that are involved in attachment to the host, blood feeding and transmission of pathogens. MicroRNAs (miRNAs) are a class of short non-coding RNAs with a length of 19-24 nucleotides. They act as regulators of gene expression by binding to their target mRNA at the post-transcriptional level and control a variety of cellular functions, including regulation of growth, metabolism, and development. The detection and characterizations of miRNAs from tick saliva may help explain the molecular mechanisms involved in the interaction between ticks, pathogens, and hosts. They may also contribute to the discovery of vaccines, which can control ticks and the pathogens they transmit. An RNA library was generated from the saliva of fed adult Haemaphysalis longicornis ticks, and it contained 17.4 million clean reads of 18-30 nucleotides. Overall, 319 known miRNAs and 1 novel miRNA were found. The 10 most abundantly expressed miRNAs present in tick saliva were miR-100_2, miR-315, miR-184_1, miR-100-5p_2, miR-5307, miR-184-3p_3, Let-7-5p_6, miR-71_5, miR-1-3p_6, and miR-10-5p_2. The miR-375, one of the abundantly expressed, was subjected to Quantitative Real-Time PCR analysis (qRT-PCR) in various tick developmental stages, as well as in different tissues isolated from adult ticks. The expression of miR-375 in different tick development stages was highest in unfed nymphs and lowest in the egg stage. In the tissues of adult ticks, miR-375 was most highly expressed in the salivary gland. To investigate the possible role of miR-375, Ant-375 was used to inhibit the miR-375. Treated group (Ant-375) had a reduced number of eggs (t(10)= 2.652, P=0.0242), eggs that were partially desiccated, and reduced egg hatchability (t(10)=2.272, P=0.044) compared to Ms-Ant and the non-injected control. This is the first study to investigate the miRNAs profile in tick saliva and the role of miR-375 in H. longicornis. The identification and characterization of miRNA in tick saliva may help to reveal the molecular mechanisms of interactions among ticks, pathogens, and hosts and suggest new vaccine strategies to control tick borne diseases.

Dataset Information

Toxicity of chlorpyrifos to the ovary of Haemaphysalis longicornis and its effect on the expression of ovarian protein

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets