Proteomics

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Investigation of Rape Bee Pollen Electrohydrodynamic Drying by Needle-To-Plate and Plate-To-Plate Electrode System


ABSTRACT: The biochemical and molecular basis of bee pollen under drying stress is not clear. In this study, metabonomics and proteomics were used to study the biochemical regulation and phenolic compound changes in the rape bee pollen of electrohydrodynamic drying. The results show that the direct contribution rate of ionic wind and electric field to drying characteristic parameters was 68.4% and 31.6% respectively. Electric field has a greater effect on the quality parameters. Specifically, compared with ionic wind, ribosome, citric acid cycle and proteasome pathway play a more positive role in regulating the abiotic and biological biochemical responses of electric field dehydration. The protein-phenolic interaction of rape bee pollen during EHD drying led to the change of free phenolic acid content and the differential accumulation of acidmetabolic characteristics such as L-phenylalanine, L-aspartic acid and L-pyroglutamic acid. In addition, TEM observation showed that EHD drying also destroyed the integrity of cell wall structure and tapetum. The results revealed the quality formation mechanism and biochemical regulation strategy of rape bee pollen during processing.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Yu Fang  

PROVIDER: PXD032251 | iProX | Mon Mar 14 00:00:00 GMT 2022

REPOSITORIES: iProX

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