Proteomic profiling reveals the molecular control of oocyte maturation

ABSTRACT: Meiotic maturation is an intricate and precisely regulated process orchestrated 32 by various pathways and numerous proteins. However, little is known about the 33 proteome landscape during oocytes maturation. Here, we obtained the temporal 34 proteomic profiles of mouse oocytes during in vivo maturation. We successfully 35 quantified 4,694 proteins from 4,500 oocytes in three key stages (GV, GVBD, and 36 MII). In particular, we discovered the novel proteomic features during oocyte 37 maturation, such as the active Skp1–Cullin–Fbox pathway and an increase in 38 mRNA-decay-related proteins. Using functional approaches, we further identified the 39 key factors controlling the histone acetylation state in oocytes and the vital proteins 40 modulating meiotic cell cycle. Taken together, our data serve as a broad resource on 41 the dynamics occurring in oocyte proteome and provide important knowledge to 42 better understand the molecular mechanisms during germ cell development.

ORGANISM(S): Mus Musculus

SUBMITTER: Xuejiang Guo  

PROVIDER: PXD034150 | iProX | Thu May 26 00:00:00 BST 2022

REPOSITORIES: iProX