Dataset Information

TMT analysis of protein expression in SVA-infected PK-15cells

ABSTRACT: we have determined changes in the host cell protein expression during SVA infection using dimethyl-labeling based quantitative proteomics.

ORGANISM(S): Sus Scrofa Domesticus

SUBMITTER: Wanzhe Yuan

PROVIDER: PXD034197 | iProX | Fri May 27 00:00:00 BST 2022

REPOSITORIES: iProX

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Publications

2B and 3C Proteins of Senecavirus A Antagonize the Antiviral Activity of DDX21 <i>via</i> the Caspase-Dependent Degradation of DDX21.

Zhao Kuan K Guo Xiao-Ran XR Liu Shuai-Feng SF Liu Xiao-Na XN Han Ying Y Wang Lu-Lu LL Lei Bai-Shi BS Zhang Wu-Chao WC Li Li-Min LM Yuan Wan-Zhe WZ

Frontiers in immunology 20220714

Senecavirus A (SVA), also known as Seneca Valley virus, is a recently discovered picornavirus that can cause swine vesicular disease, posing a great threat to the global swine industry. It can replicate efficiently in cells, but the molecular mechanism remains poorly understood. This study determined the host's differentially expressed proteins (DEPs) during SVA infection using dimethyl labeling based on quantitative proteomics. Among the DE proteins, DDX21, a member of the DEAD (Asp-Glu-Ala-Asp ...[more]

PMID: 35911774

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Project description:Leishmania donovani, an intracellular protozoan parasite, is the causative agent of visceral leishmaniasis or kala-azar, the most severe form of leishmaniasis in humans. To date, our understanding of the molecular mechanisms associated with the pathogenicity of Leishmania infection is still limited. RNA interference—collectively RNA silencing pathways—participates in the regulation of various biological processes in most eukaryotic cells. Complexes of Argonaute proteins with small RNAs are core components of the RNA interference system and play a key role in silencing gene expression. It is becoming increasingly clear that several intracellular pathogens target host cell RNA interference pathways to promote their survival. In this study, we investigated the potential role of host macrophage Argonautes in Leishmania pathogenesis. Western blot analysis showed that protein abundance of infected macrophage Argonaute 1 (Ago1) was selectively and significantly higher than that of non-infected control at 24 h post-infection, suggesting that Ago1 plays a role in pathogenicity. In fact, siRNA-mediated downregulation of Ago1 enhanced Leishmania clearance from infected host cells, linking macrophage Ago1 to Leishmania virulence. To investigate the mechanisms of host Ago1 in Leishmania pathogenesis, a stable isotope labeling by amino acids in cell culture (SILAC)-based whole proteome approach was employed, which showed that expression of several previously reported Leishmania pathogenesis-related proteins were dependent on the level of macrophage Ago1. Moreover, the proteomic-based detailed biochemical analysis showed that Leishmania modulated host RNA-induced silencing complex (RISC) composition during infection, strongly suggesting macrophage RISC targeting. Strikingly, Leishmania proteins were detected as part of host RISC in infected cells. Together, our results demonstrate that Leishmania targets host RNA interference machinery to promote its survival inside the host macrophage.

Dataset Information

TMT analysis of protein expression in SVA-infected PK-15cells

Publications

2B and 3C Proteins of Senecavirus A Antagonize the Antiviral Activity of DDX21 <i>via</i> the Caspase-Dependent Degradation of DDX21.

Similar Datasets

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