Proteomics

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Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics


ABSTRACT: This repository is related to the work "Benchmarking commonly usedsoftware suites and analysis workflows for DIA proteomics and phosphoproteomics". The following files are stored here: 1. four MS datasets generated in this work, including two two-species benchmark datasets acquired on QE HF and timsTOF Pro, and two TNF-alpha induced phosphoproteomics datasets acquired on QE HF-X and timsTOF Pro; 2. all spectral libraries used in this work; 3. raw MS data search reports with analysis logs included if software reported; 4. preprocessed MS data search reports for any result from HF and TIMS DIA benchmark datasets; 5. FASTAs used in this work; 6. a description file (Description_of_iProX_and_public_data.xlsx) for the stored files in this repository and other public data used in this work. Note: 3 and 4 are packed into one zip file for each data search experiment.

ORGANISM(S): Homo Sapiens Saccharomyces Cerevisiae S288c Mus Musculus

SUBMITTER: Wenqing Shui  

PROVIDER: PXD034709 | iProX | Thu Dec 08 00:00:00 GMT 2022

REPOSITORIES: iProX

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Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics.

Lou Ronghui R   Cao Ye Y   Li Shanshan S   Lang Xiaoyu X   Li Yunxia Y   Zhang Yaoyang Y   Shui Wenqing W  

Nature communications 20230106 1


A plethora of software suites and multiple classes of spectral libraries have been developed to enhance the depth and robustness of data-independent acquisition (DIA) data processing. However, how the combination of a DIA software tool and a spectral library impacts the outcome of DIA proteomics and phosphoproteomics data analysis has been rarely investigated using benchmark data that mimics biological complexity. In this study, we create DIA benchmark data sets simulating the regulation of thou  ...[more]

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