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Photoactivation-dependent proximity labeling (PDPL) MS

ABSTRACT: we report a photoactivation-dependent proximity labeling (PDPL) method designed by genetically attaching photosensitizer protein miniSOG to a protein of interest (POI). Triggered by blue light, singlet oxygen is generated, enabling temporally-resolved chemical probe labeling.

ORGANISM(S): Homo Sapiens

SUBMITTER: Gang Li

PROVIDER: PXD034811 | iProX | Wed Jun 22 00:00:00 BST 2022

REPOSITORIES: iProX

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Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling.

Zhai Yansheng Y Huang Xiaoyan X Zhang Keren K Huang Yuchen Y Jiang Yanlong Y Cui Jingwei J Zhang Zhe Z Chiu Cookson K C CKC Zhong Weiye W Li Gang G

Nature communications 20220820 1

Enzymatic-based proximity labeling approaches based on activated esters or phenoxy radicals have been widely used for mapping subcellular proteome and protein interactors in living cells. However, activated esters are poorly reactive which leads to a wide labeling radius and phenoxy radicals generated by peroxide treatment may disturb redox-sensitive pathways. Herein, we report a photoactivation-dependent proximity labeling (PDPL) method designed by genetically attaching photosensitizer protein ...[more]

PMID: 35987950

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