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Study of interacting proteins of FOXO1 using TurboID-based proximity labeling technology

ABSTRACT: Based on the TurboID proximity labeling technology, the interacting proteins of FOXO1 were studied and divided into three groups: blank group U251 cells, FOXO1-U251 overexpressing cells, and FOXO1-U251 cells treated with LPS. The three groups of cells were affinity purified by streptomycin magnetic beads and identified by protein silver staining, and then sent to the company for label-free protein analysis to obtain the original data of the three groups of protein spectra.

ORGANISM(S): Homo Sapiens

SUBMITTER: Chao Liu

PROVIDER: PXD036171 | iProX | Fri Aug 19 00:00:00 BST 2022

REPOSITORIES: iProX

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Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology.

Su Yanting Y Guo Yuanyuan Y Guo Jieyu J Zeng Ting T Wang Ting T Liu Wu W

BMC genomics 20230324 1

<h4>Background</h4>Protein‒protein interactions (PPIs) are the foundation of the life activities of cells. TurboID is a biotin ligase with higher catalytic efficiency than BioID or APEX that reduces the required labeling time from 18 h to 10 min. Since many proteins participate in binding and catalytic events that are very short-lived, it is theoretically possible to find relatively novel binding proteins using the TurboID technique. Cell proliferation, apoptosis, autophagy, oxidative stress and ...[more]

PMID: 36964488

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