Dataset Information

Proteomic analysis using TMT technology reveals the effect of Polyascus gregaria on hepatopancreatic oxidative stress and apoptosis in the male Chinese mitten crab (Eriocheir sinensis)

ABSTRACT: Differentially expressed proteins in the E. sinensis hepatopancreas were detected after P. gregaria infection using tandem mass tag technology. Gene Ontology and Kyoto En-cyclopedia of Genes and Genomes enrichment analyses showed that the differentially expressed proteins were closely related to oxidative stress and apoptosis. Pathological sections and liver bi-ochemical indicators indicated that the liver was damaged after P. gregaria infection in the hepatopancreas of E. sinensis. Furthermore, the hepatopancreas tissue showed increased oxidative stress and apoptosis in the liver cells. This study provides a theoretical basis for understanding the pathological mechanism of P. gregaria infection in E. sinensis hepatopancreas and may guide future research directions for the prevention and treatment of P. gregaria infection.

ORGANISM(S): Polyascus Gregaria Eriocheir Sinensis

SUBMITTER: Xiaodong Li

PROVIDER: PXD038210 | iProX | Fri Nov 18 00:00:00 GMT 2022

REPOSITORIES: iProX

ACCESS DATA

Similar Datasets

Project description:Spiroplasma eriocheiris, has been identified as a novel lethal pathogen of Eriocheir sinensis tremor disease (TD), one neurological disease with typically paroxysmal tremors of the pereiopod. This pathogen infected and multiplied in the hemocytes of E. sinensis as the first target cells, and then follow the blood circulation to infect the crab other tissues. S. eriocheiris infected the nerves tissue is the directly reason of TD. But the pathogenic mechanism of TD was still few known. Firstly, in the current study, the phosphoproteomic changes of E. sinensis thoracic ganglion after S. eriocheiris infection were obtained. KEGG pathway analysis show Wnt signaling pathways was restrained, corresponding many nervous system development and signal transmission pathway also destroyed. Base on the identified modified sequence, several peptides (GSK3β, SYN, VAMP and SNAMP-25) were selected to synthesize chemically and prepare phosphorylated antibodies. The differentially expressed phosphorylated proteins GSK3β-pSer9, VAMP-pSer72, SNAP25-pSer102, and SYN-pSer134 in thoracic ganglion were significantly down-regulated verified by immunohistochemistry and western blot, this results are similar with the phosphoproteomic. By qRT-PCR, western blot, RNA interference and inhibitor experiments, when the S. eriocheiris infected the hemocytes of crab, the GSK-3β and β-Catenin in Wnt-β-Catenin pathway were restrained similar in the thoracic ganglion. The S. eriocheiris can restricts the hemocytes Wnt-β-Catenin pathway to help itself infection no matter in vivo or in vitro. Neurotransmitter metabolite analysis showed that four kinds of neurotransmitters (5-Hydroxy-L-tryptophan, Serotonin, Acetylcholine and γ-Amino-butyric acid) in thoracic ganglion were metabolic disorders in E. sinensis thoracic ganglion after S. eriocheiris infection. The present work could serve as a basis for understanding the role of Wnt signaling pathway in the process of S. eriocheiris invasion E. sinensis hemocytes and causing paroxysmal tremors of E. sinensis the pereiopod.

Dataset Information

Proteomic analysis using TMT technology reveals the effect of Polyascus gregaria on hepatopancreatic oxidative stress and apoptosis in the male Chinese mitten crab (Eriocheir sinensis)

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets