Dataset Information

Multi-omics revealed the difference in epididymal cauda expression in yak (Bos grunniens) before and after sexual maturation

ABSTRACT: Normal epididymis development is the basis of male reproduction, and epididymis is a crucial site where sperm maturation occurs. Although epididymis has been studied through multiple omics methods, combined omics has not been used to present the differential genes in the epididymal cauda of yaks before and after sexual maturity. We here provided the landscape maps of differential genes and proteins obtained through RNA-seq and proteomics technology, and searched for potential key genes, including TGFBI, COL1A1, COL1A2, COL3A1, COL12A1, SULT2B1, KRT19, and NPC2, through integration analysis. In separate and combined enrichment analyses, genes mainly related to cell growth, differentiation and adhesion, sperm maturation, and immune defense were differentially expressed. The involved pathways included extracellular matrix (ECM)–receptor interaction, protein differentiation and absorption, lysosomes, and estrogen signaling pathway. When the sequencing results were verified through qRT-PCR and 4D-PRM, they were found to be consistent. In addition, after the key genes were identified, abnormal expression of these genes was found to possibly cause the retardation of cauda epididymis development and abnormal sperm function in yaks. In conclusion, we provide useful clues for the development of epididymal cauda of yak, sperm maturation, and screening of key genes involved in reproductive regulation of male yak through separate and combined analyses.

ORGANISM(S): Bos Grunniens

SUBMITTER: Xian Guo

PROVIDER: PXD039871 | iProX | Sat Feb 04 00:00:00 GMT 2023

REPOSITORIES: iProX

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Publications

Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity.

Ding Ziqiang Z Xiong Lin L Wang Xingdong X Guo Shaoke S Cao Mengli M Kang Yandong Y La Yongfu Y Bao Pengjia P Pei Jie J Guo Xian X

Animals : an open access journal from MDPI 20230415 8

Epididymis development is the basis of male reproduction and is a crucial site where sperm maturation occurs. In order to further understand the epididymal development of yak and how to regulate sperm maturation, we conducted a multi-omics analysis. We detected 2274 differential genes, 222 differential proteins and 117 co-expression genes in the cauda epididymis of yak before and after sexual maturity by RNA-seq and proteomics techniques, which included TGFBI, COL1A1, COL1A2, COL3A1, COL12A1, SU ...[more]

PMID: 37106918

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Project description:<p>The epididymis is the primary organ responsible for sperm maturation, with its caudal region serving as the primary storage site for mature sperm. The unique small molecule metabolites present in the cauda epididymal fluid not only facilitate the long-term storage of sperm but also ensure their structural and functional integrity prior to fertilization. Consequently, an in-depth investigation into the composition and functions of these metabolites in the cauda epididymal fluid is essential for elucidating the regulatory mechanisms governing sperm maturation and fertilization. Yaks, as livestock unique to the Qinghai-Tibet Plateau, hold significant scientific and economic value. Compared to cattle, male yaks exhibit poorer reproductive performance, characterized by reduced sperm motility, elevated sperm abnormality rate, and lower artificial insemination conception rates. To investigate the relationship between sperm motility defects in yaks and the microenvironment during their maturation, this study collected cauda epididymal fluid from yaks and cattle for untargeted metabolomic sequencing. The results indicated that 1,098 and 1,297 kinds of metabolites annotated by the Human Metabolome Database (HMDB) database were identified in yak and cattle cauda epididymal fluid, respectively, using positive and negative ion modes. In both modes, the three categories containing the most types of metabolites were organic acids and derivatives, organoheterocyclic compounds, and lipids and lipid-like molecules. Within the lipid metabolites, fatty acids and conjugates were the most prevalent in both modes. In the positive ion mode, compared to cattle cauda epididymal fluid, 79 metabolites were significantly upregulated and 212 were significantly downregulated in yak cauda epididymal fluid. In the negative ion mode, compared to cattle cauda epididymal fluid, 110 metabolites were significantly upregulated and 230 metabolites were significantly downregulated in yak cauda epididymal fluid. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment results showed that the most significantly enriched pathway in the positive ion mode was the biosynthesis of amino acids, while in the negative ion mode, it was ABC transporters. Among the significant differentially expressed metabolites, 18 have been associated with sperm mobility, maturation or function. The functions of these metabolites involve regulation of oxidative stress, sperm capacitation, spontaneous acrosome reaction, mitochondrial energy metabolism, mRNA methylation, and flagellar motility. Taken together, these findings establish a foundational dataset and offer novel perspectives for elucidating the molecular mechanisms underlying yak sperm maturation, enhancing sperm motility, optimizing reproductive techniques, and ultimately improving reproductive performance.</p>

Dataset Information

Multi-omics revealed the difference in epididymal cauda expression in yak (Bos grunniens) before and after sexual maturation

Publications

Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity.

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