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Study on the infection mechanism of Verticillium dahliae on potato regulated by the viruence-related pigment biosynthesis protein Vayg1

ABSTRACT: Verticillium wilt of potato caused by Verticillium dahliae, also called potato early dying disease, is an important factor limiting potato production in the world. Currently, the poor understanding of the pathogenic mechanism of Verticillium dahliae has led to increasingly severe challenges in the effective control of Verticillium wilt. Many studies have shown that pigment biosynthesis related proteins are virulence factors of Verticillium dahliae, while the regulatory mechanisms are still unknown. In order to elucidate the pathogenic mechanism regulated by the virulence related pigment biosynthesis protein Vayg1 in Verticillium dahliae, proteomic analysis will be used to judge the Vayg1 deletion mutant and the wild-type strain during the infection of potato to explore the Vayg1 involved pathogenic protein network, aiming to identify and understand relevant pathogenic pathways to provide an important support for deeper understanding of the pathogenic mechanism of the pathogen. The key proteins related to virulence and molecular networks that will be obtained in this study could provide new targets for the control of Verticillium wilt and new insights for the improvement of disease management.

ORGANISM(S): Solanum Tuberosum Verticillium Dahliae

SUBMITTER: Fan Rong

PROVIDER: PXD040131 | iProX | Wed Feb 15 00:00:00 GMT 2023

REPOSITORIES: iProX

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Project description:Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. The PCM is a systemic disease caused by the thermo dimorphic fungus Paracoccidioides spp, which can exist as mycelia and yeast forms. The mycelia form can be found in environment at temperatures between 18 °C and 25 °C and produces spores or conidia. The PCM infection route occurs by the inhalation of conidia. Once in the lungs, these particles are converted into yeasts pathogenic form. Until now, metabolic aspects and virulence factors related to Paracoccidioides lutzii infective propagule are little understood. In this concern, a global proteomic study of P. lutzii conidia was performed. For conidia production, the mycelia of the isolate Pb01 were cultured in potato agar medium during 90 days at 18 °C. Obtained conidia were collected and purified. The proteins were extracted and subjected to tryptic digestion followed by identification by NanoUPLC-MSE approach. We identified a total of 242 proteins in P. lutzii conidia which were subjected to in silico functional analysis Proteins putatively acting as adhesins, such as GAPDH, enolase, and the glycoprotein β-1,3-glucanosyltransferase (gel2) were identified during our analysis. We also identified proteins related to signal transduction pathways, such as Ras and RhoA GTPases, previously demonstrated to be required for morphologic changes in pathogenic fungi. Proteins related to evasion, defense and virulence were also identified. Proteins acting during temperature shifts or oxidative stress provided by the host environment such as HSP90, catalase, mitochondrial peroxiredoxin Prx1 were identified. It were also identified proteins related to energy production and protein biosynthesis, which can provide important aspects to survival of this resting cells. These results highlight that P. lutzii conidia contain proteins that can contribute to its maintenance in the environment and also molecules related to host-pathogen interactions.

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Study on the infection mechanism of Verticillium dahliae on potato regulated by the viruence-related pigment biosynthesis protein Vayg1

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