Spatiotemporally direct capturing global substrates of post-translational modifying enzymes in living cells

ABSTRACT: Methods for direct capturing global substrates of protein-modifying enzymes in living cells are with many challenges, and yet largely unexplored. Here, we report a strategy to directly capture substrates of protein-modifying enzymes via PTM-acceptor residue crosslinking in living cells, enabling global profiling of substrates of PTM-enzymes and validation of PTM-sites in a straightforward manner.

ORGANISM(S): Escherichia Coli

SUBMITTER: Minjia Tan  

PROVIDER: PXD040318 | iProX | Wed Feb 22 00:00:00 GMT 2023

REPOSITORIES: iProX