Large-scale Quantitative Proteomics Analysis During Different Stages of Somatic Embryogenesis in Larix olgensis

ABSTRACT: Larix olgensis is an economically important tree species native to northeastern China. Its somatic embryogenesis (SE) system is both efficient and stable, and this permits varieties with desirable qualities to be reproduced rapidly. Here, isobaric labeling via tandem mass tags was used to conduct a large-scale quantitative proteomics analysis of proteins in three critically important stages of SE in L. olgensis: the primary embryogenic callus, single embryo, and cotyledon embryo. We identified 6,269 proteins, including 176 shared differentially expressed proteins across the three groups. Many of these proteins were involved in glycolipid metabolism, hormone response/signal transduction, cell synthesis and differentiation, and water transport; proteins involved in stress resistance and secondary metabolism, as well as transcription factors, play key regulatory roles in SE. The results of this study provide new insights into the key pathways and proteins involved in SE in Larix. Our findings have implications f

ORGANISM(S): Larix

SUBMITTER: Ying Gai  

PROVIDER: PXD040378 | iProX | Fri Feb 24 00:00:00 GMT 2023

REPOSITORIES: iProX