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Proteomics data of Fusobacterium nucleatum outer membrane vesicles

ABSTRACT: This is the raw proteomics data of Fusobacterium nucleatum outer membrane vesicles

ORGANISM(S): Fusobacterium Nucleatum Subsp. Polymorphum Atcc 10953

SUBMITTER: Juan Li

PROVIDER: PXD040509 | iProX | Tue Feb 28 00:00:00 GMT 2023

REPOSITORIES: iProX

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Project description:At present, the function of Fusobacterium nucleosocci outer membrane vesicles under normal intestinal environmental pH conditions has been mainly studied, but there are few studies related to the function of Fusobacterium nucleosius outer membrane vesicles under cancer microenvironment pH conditions. The purpose of this study was to pioneer the comparative analysis of the composition of Fusobacterium nucleosibacterium outer membrane vesicle proteins under normal intestinal environmental pH conditions and cancer microenvironment pH conditions, and to find out the virulence factor protein of Fusobacterium nucleosocci outer membrane vesicles under cancer microenvironment pH conditions. To do this, we adjusted the initial pH of TSPC medium to around 6.0 using acidic cysteine to mimic the cancer microenvironment pH conditions. We combined ultracentrifugation and density gradient centrifugation to separate and extract Fusobacterium nucleosi's outer membrane vesicles from two pH environments, and used TMT quantitative proteomics to identify proteins in outer membrane vesicles. A total of 991 proteins were identified, compared with normal outer membrane vesicles, 306 proteins in the outer membrane vesicles in the tumor environment were up-regulated and 360 proteins were down-regulated, and some of the up-regulated proteins of the out-membrane vesicles in the tumor environment were virulence factor proteins, such as multiple pentatype autotransporters and MORN2 domain proteins, and the functions of these proteins were mainly related to adhesion and invasion. In addition, the down-regulation of enzyme levels involved in butyrate metabolism in the outer membrane vesicles of Fusobacterium nucleosionae under the condition of cancer microenvironment pH inhibits the butyric acid production capacity of Fusobacterium nucleobacteria, resulting in a decrease in butyric acid production capacity, which may promote the development of colorectal cancer. In conclusion, quantitative proteomics comparative analysis has increased our understanding of Fusobacterium nucleosom outer membrane vesicle virulence protein in the tumor environment.

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Proteomics data of Fusobacterium nucleatum outer membrane vesicles

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