Functional differences of experimental arthritis splenic macrophages in DA and DAMut rats are revealed by quantitative proteomics
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ABSTRACT: Rheumatoid arthritis (RA) is a devastating chronic inflammatory disorder affecting 0.5-1.0% of adults worldwide and is characterized by autoimmune reactivity and persistent active inflammation with concurrent tissue destruction. Animal models of RA provide an opportunity to understand disease pathogenesis and develop new therapeutic strategies . The DA inbred rat is commonly used in autoimmunity research due to its high susceptibility to several chronic inflammatory disorders such as arthritis and experimental autoimmune encephalomyelitis (EAE). The experimental arthritis induced by pristane, the arthritogenic component in mineral oil, in the DA rat can be classified with RA criteria, including a chronic relapsing disease course 5, 6. We have identified a mutation DA rat and the mutation plays a completely protective function in DA rats against EAE and pristane-induced arthritis (PIA). Macrophages are an essential source of pro-inflammatory cytokines in chronic inflammation and the progressive destruction of synovial joints. To elucidate the molecular mechanism by which the mutation regulates macrophage activation, we made quantitative mass spectrometry on the four groups of splenic macrophages: DA-control, DAMut-control, DA-PIA, DAMut-PIA.
ORGANISM(S): Rattus Norvegicus
SUBMITTER: Liesu Meng
PROVIDER: PXD041317 | iProX | Tue Apr 04 00:00:00 BST 2023
REPOSITORIES: iProX
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