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TDRD6 IP-MS data in mouse testis protein lysates

ABSTRACT: We immunoprecipitated endogenous TDRD6 from testis protein lysates of 28-day-old wild-type mice using our home-made TDRD6 antiserum. No TDRD6 protein was identified in Tdrd6-mutant mice carrying the p.G615X (GGG to TGA) variant. Thus, Tdrd6-mutant mice were utilized as negative control. A high-throughput technology, LC/MS–MS (MS), was applied to identify the candidate interacting proteins of TDRD6.

ORGANISM(S): Mus Musculus

SUBMITTER: Suren Chen

PROVIDER: PXD042162 | iProX | Thu May 11 00:00:00 BST 2023

REPOSITORIES: iProX

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Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice.

Bi Xinying X Jin Huijuan H Wan Feng F Xia Yanqing Y Guo Haibin H Chen Suren S Wang Binbin B

Journal of cellular and molecular medicine 20240901 18

Oligo-astheno-teratozoospermia (OAT) is a common cause of male infertility, but the genetic basis of most OAT cases is still unknown. Here, one homozygous loss-of-function (LOF) variant in TDRD6, c.G1825T/p.Gly609X, was identified in an infertile patient with severe OAT by whole-exome sequencing (WES) and Sanger confirmation. Furthermore, Tdrd6-mutant mice (p.Gly615X; equivalent to p.Gly609X in human TDRD6) were generated. Remarkably, the Tdrd6-mutated mice mimicked the severe OAT symptoms of th ...[more]

PMID: 39331689

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