Proteome responses of tomato root exposed to cadmium stress
Ontology highlight
ABSTRACT:
ORGANISM(S): Solanum Lycopersicum
SUBMITTER: 
Chaochao Liu
PROVIDER: PXD042523 | iProX | Fri May 26 00:00:00 GMT+01:00 2023
REPOSITORIES: iProX
Similar Datasets
Project description:A first insight into the effects of cadmium exposure on the phosphoproteome of tomato plants by performing a comparative analysis of tomato genotypes with contrasting cadmium tolerance.
2021-08-11 | PXD024021 | Pride
Project description:Excess levels of Al3+ become highly harmful to plant roots as the toxic ions bind to the surface of root-tips. Plants can acquire tolerance to stress after multiple generations of exposure to stress conditions. After four consecutive generations of Al treatments (G4Al+ seeds), tomato plants appeared more tolerant to the stress condition. This project used laser capture microdissection (LCM) technology to isolate the Al-sensitive epidermal and outer cortical cells of root-tips. Tandem mass tag (TMT) proteomics analysis was conducted to identify proteomics changes related to the physiological properties in root growth under Al treated conditions.
2022-02-14 | PXD018234 | Pride
Project description:Prunus mongolica is a relict xerophytic shrub with ecological importance for desert ecosystem stability and vegetation restoration in arid and semi-arid regions. However, the physiological and molecular mechanisms underlying its responses to combined alkali-salt and cadmium stress remain unclear. This study aimed to investigate the regulatory role of exogenous gamma-aminobutyric acid (GABA) in improving stress tolerance in Prunus mongolica under alkali-salt stress, cadmium stress, and their combined stress. Seedlings were subjected to eight treatments, including control, GABA alone, alkali-salt stress, cadmium stress, combined alkali-salt and cadmium stress, and the corresponding GABA-treated stress groups. Germination traits, seedling growth, physiological indices, antioxidant responses, osmotic adjustment, photosynthetic pigments, and transcriptomic profiles were analyzed to evaluate the effects of GABA at multiple biological levels. Alkali-salt, cadmium, and combined alkali-salt and cadmium stress markedly inhibited seed germination and seedling growth, with the combined stress causing the most severe damage. Stress treatments promoted reactive oxygen species accumulation, lipid peroxidation, photosynthetic inhibition, and reductions in water status and root activity. Exogenous GABA alleviated these stress-induced effects by improving germination performance, seedling growth, chlorophyll accumulation, root activity, and relative water content, while reducing oxidative damage. GABA also enhanced osmotic adjustment by increasing proline and soluble sugar contents and strengthened antioxidant defense through the activation of superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, and glutathione reductase. Transcriptome analysis showed that GABA modulated stress-responsive genes associated with MAPK signaling, plant hormone signal transduction, plant-pathogen interaction, peroxisome, glutathione metabolism, photosynthesis, carbon metabolism, and other primary metabolic processes. These results suggest that exogenous GABA enhances stress tolerance in Prunus mongolica by coordinating redox homeostasis, osmotic regulation, stress signaling, photosynthetic recovery, and metabolic reprogramming under single and combined alkali-salt and cadmium stresses.
2026-07-03 | GSE337475 | GEO
Project description:The purpose of this dataset is to generate a transcriptomic series of staged non-induced lateral root intiation in M82 tomato. Sections of the primary roots containing lateral roots at 5 different developemental stages (staged by their anatomy and expression of the auxin response marker DR5) were collected.
2022-03-04 | GSE159050 | GEO
Project description:Cadmium (Cd) contamination severely inhibits plant growth and development by inducing oxidative damage and disrupting cellular homeostasis. Indole-3-acetic acid (IAA) has been reported to enhance plant tolerance to abiotic stresses; however, the molecular mechanisms underlying IAA-mediated Cd tolerance remain largely unclear. In this study, wild-type tomato plants (Solanum lycopersicum L.) were treated with Cd stress and exogenous IAA, and leaf samples were subjected to transcriptome sequencing to investigate gene expression changes associated with IAA-induced Cd tolerance. The results provide transcriptomic insights into the regulatory mechanisms of IAA in alleviating Cd toxicity in tomato and contribute to understanding the role of hormone-mediated stress adaptation in plants.
2026-05-28 | GSE332769 | GEO
Project description:rs06-03_cd-mirna - cadmium-mirna - trancriptional and post-transcriptional response to cadmium. - After 5 days of grown in a fresh medium (5% PCV at day0), CdCl2 was added to tested cells (Cad) to a final concentration of 200uM. Nothing was added to control cells (Tem). After 12 and 24 hours of growth +/- cadmium, cells were harvested and frozen in liquid nitrogen.
2012-03-25 | GSE36627 | GEO
Project description:The aim of this work was to study the effects of Fe and Mn deficiencies and Mn toxicity on the protein profile of the root of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published. The high-throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato root. Protein identification was carried out using the Mascot search engine and the non-redundant databases NCBInr and ITAG v2.3.
2018-08-06 | PXD008326 | Pride
Project description:We performed a transcriptomic analysis on two tomato genotypes, M82 and Tondo, in response to a PEG-mediated osmotic treatment, mimicking water stress. The analysis was conducted separately on leaves and roots to characterize the specific response of these two organs. A total of 6,267 differentially expressed transcripts related to osmotic stress response was detected. The construction of gene co-expression networks defined the metabolic and signaling pathways of the common and specific responses of leaf and root. The common response was characterized by ABA-dependent and ABA-independent signaling pathways, and by the interconnection between ABA and JA signaling. The root specific response concerned genes involved in cell wall metabolism and remodeling, whereas the leaf specific response was principally related to leaf senescence and ethylene signaling. The transcription factors representing the hubs of these regulatory networks were identified. Some of them have not yet characterized and can represent novel candidates for tolerance. Finally, several genes showing a genotype-specific expression regulation in response to the treatment were detected. These genes may be involved in the different sensitivity to the osmotic treatment of the two tomato genotypes. In conclusion, this work shed new light on the regulatory networks occurring in tomato leaf and root under osmotic stress and set the base for an in-depth characterization of novel stress-related genes, that may represent potential candidates for improving tolerance to water stress in tomato.
2023-05-15 | GSE224629 | GEO
Project description:Sl2183 is an updated version of the previous tomato metabolic model (iHY3410), with additional reactions and metabolites, IDs converted into the BiGG nomenclature and biomass reactions for leaf, stem and root, allowing to generate a multi-organ model (see Gerlin et al., Plant Physiol. for additional information).
2022-04-07 | MODEL2111120001 | BioModels
Project description:We used Affymetrix microarrays to profile the Dapnia pulex miRNA expression under Cadmium exposure and identified Cadmium-responsive miRNAs
2015-10-15 | GSE68481 | GEO