Proteomics

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High-Throughput Intact Glycopeptide Quantification Strategy with Targeted-MS (HTiGQs-Target) Reveals Site-Specific IgG N-Glycopeptides as Biomarkers for Hepatic Disorder Diagnosis and Staging

ABSTRACT: We developed a high-throughput targeted quantification strategy for intact glycopeptides (HTiGQs-Target) to diagnose and differentiate the malignancy of liver diseases. The method involves two-step labeling. This step derivatization makes the same parent ions in different samples produce sufficient mass differences in primary mass spectrometry detection, and finally expands the sample detection throughput to 20 (2*10) samples per run. We used this method to analyze serum samples from patients with different types and stages of liver diseases, and found some glycopeptide features with diagnostic and differential significance. This project provides a new and efficient proteomics method for early diagnosis and treatment of liver diseases.

ORGANISM(S): Homo Sapiens

SUBMITTER: Haojie Lu  

PROVIDER: PXD042528 | iProX | Tue May 30 00:00:00 BST 2023

REPOSITORIES: iProX

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High-throughput intact Glycopeptide quantification strategy with targeted-MS (HTiGQs-target) reveals site-specific IgG N-glycopeptides as biomarkers for hepatic disorder diagnosis and staging.

Liu Xuejiao X   Fu Bin B   Chen Jierong J   Sun Zhenyu Z   Zheng Dongdong D   Li Zhonghua Z   Gu Bing B   Zhang Ying Y   Lu Haojie H  

Carbohydrate polymers 20231016

Liver disease is one of the leading causes of global mortality, and identifying biomarkers for diagnosing the progression of liver diseases is crucial for improving its outcomes. Targeted mass spectrometry technology is a powerful tool with unique advantages for verifying biomarker candidates and clinical applications. It is particularly useful in validating protein biomarkers with post-translational modifications, eliminating the need for site-specific antibodies. Especially, targeted mass spec  ...[more]

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