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COVID-19 increases extracorporeal coagulation during hemodialysis through upregulation of vWF/FBNL5 signaling

ABSTRACT: Introduction: COVID-19 has been shown to increase the risk of extracorporeal coagulation during hemodialysis in patients, but the mechanism is unclear. This study aims to investigate the effect and mechanism of COVID-19 on the risk of extracorporeal coagulation during hemodialysis. Methods: A retrospective analysis was performed on the extracorporeal coagulation status of 339 hemodialysis patients at our hemodialysis center before and after COVID-19 infection. Protein spectroscopy was used to analyze blood composition differences between asymptomatic/mild and severe/critical patients after infection. Results: In comparison to the pre-COVID-19 infection period, a notable increase in the risk of extracorporeal coagulation during hemodialysis after infection has been observed. The grade of coagulation was directly proportional to the severity of infection symptoms, especially in severe and critically symptoms patients. In addition, proteomic analysis has demonstrated that in severe/critical cases, the coagulation cascade reaction, platelet activation, inflammation, and oxidative stress-related signals were significantly amplified as compared to asymptomatic/mild cases. Notably, the coagulation-related signaling pathway vWF/FBNL5 was significantly upregulated. Conclusions: In conclusion, this study revealing the potential role of vWF/FBNL5 in COVID-19-induced coagulation, which can have severe implications for patients undergoing hemodialysis. The findings highlight the critical importance of early initiation of anticoagulant therapy for COVID-19 patients, particularly those undergoing hemodialysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yuxin Yin

PROVIDER: PXD044377 | iProX | Thu Aug 03 00:00:00 BST 2023

REPOSITORIES: iProX

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COVID-19 increases extracorporeal coagulation during hemodialysis associated with upregulation of vWF/FBLN5 signaling in patients with severe/critical symptoms.

Yang Guang G Shan Hui H Wu Dibin D Li Sanmu S Lai Zhiwei Z Zheng Fengping F Xiong Zibo Z Xiong Zuying Z Diao Yuhan Y Shan Ying Y Chen Yun Y Wang Aihong A Liang Wei W Yin Yuxin Y

BMC infectious diseases 20240422 1

<h4>Background</h4>COVID-19 has been shown to increase the risk of extracorporeal coagulation during hemodialysis in patients, but the underlying mechanism remains unclear. This study aimed to investigate the effect and mechanism of COVID-19 on the risk of extracorporeal coagulation in patients with chronic kidney disease undergoing hemodialysis.<h4>Methods</h4>A retrospective analysis of the extracorporeal coagulation status of 339 hemodialysis patients at our center before and after COVID-19 i ...[more]

PMID: 38649864

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Project description:Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has provoked, up to date, more than 5 million deaths worldwide. While asymptomatic individuals are responsible of many potential transmissions, the difficulty to identify and isolate them at the high peak of infection constitutes still a real challenge in the actual pandemic. Moreover, apart from the effect over the respiratory system, SARS-CoV-2 provokes severe vascular damage and thromboembolic events in critical COVID-19 patients, deriving in many related deaths and long-hauler symptoms. Understanding how these processes are triggered in response to the virus as well as the potential sequelae at the long term, even in asymptomatic donors, becomes essential. Methods: We have evaluated, by application of a proteomics-based quantitative approach, the effect of serum of COVID-19 asymptomatic individuals over circulating angiogenic cells (CACs). Thus, healthy CACs were incubated ex-vivo with the serum of either COVID-19 negative (PCR-/IgG-, n:8) or COVID-19 positive asymptomatic donors, at different infective stages: PCR+/ IgG- (n:8) and PCR-/IgG+ (n:8). Also, a label free quantitative approach was applied to identify and quantify protein differences between these serums. Results: Our results confirmed that SARS-CoV-2 promotes changes at the protein level in the serum of infected asymptomatic individuals, mainly correlated with altered coagulation and inflammatory processes (Fibrinogen, Von Willebrand Factor, Thrombospondin-1). At the cellular level, proteins like ICAM-1, TLR2 or Ezrin/Radixin were only up-regulated in CACs treated with the serum of asymptomatic patients at the highest peak of infection (PCR+/IgG-), but not with the serum of PCR-/IgG+ individuals. Many of these proteins correlate with the initial response of the endothelium against the virus. Conclusions: The incubation ex vivo of CACs with the serum of asymptomatic COVID-19 donors at different stages of infection promoted protein changes representative of the endothelial dysfunction and inflammatory response that occur after viral infection, together with activation of the coagulation process. The current approach constitutes an optimal model to study the response of vascular cells to SARS-CoV-2 infection, as well as an alternative to test potential inhibitors targeting either the virus entry pathway or molecules involved in the immune responses following SARS-CoV-2 infection.

Dataset Information

COVID-19 increases extracorporeal coagulation during hemodialysis through upregulation of vWF/FBNL5 signaling

Publications

COVID-19 increases extracorporeal coagulation during hemodialysis associated with upregulation of vWF/FBLN5 signaling in patients with severe/critical symptoms.

Similar Datasets

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