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Plasma Proteome Profiling Identified thrombospondin-1 and lactoferrin as Biomarkers of Relapsed Multiple Myeloma

ABSTRACT: In this study, serum from bone marrow and peripheral blood was collected from patients with newly diagnosed MM (NDMM), RRMM, and MM in remission as well as healthy controls. Mass spectrometry-based tandem mass tag-labeled quantitative proteomics was applied to measure the levels of more than 1000 proteins in the serum. The results revealed the low expression of thrombospondin-1 (THBS1) and lactoferrin (LTF) in the bone marrow serum of patients with RRMM, indicating that these proteins are associated with MM relapse. The enzyme-linked immunosorbent assay (ELISA) results also revealed that THBS1 and LTF were downregulated in the bone marrow serum of patients with RRMM compared with the findings in patients with NDMM, and their levels in peripheral blood serum in the RRMM group were much lower than those in the NDMM, MM in remission, and healthy control groups. Based on the proteomics and ELISA data, the protein signature THBS1/LTF could contribute to the prediction of MM relapse.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaoxiao Wu

PROVIDER: PXD045583 | iProX | Fri Sep 22 00:00:00 BST 2023

REPOSITORIES: iProX

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