Dataset Information

Phosphoproteome of Monascus purpureus

ABSTRACT: MpSTE1 mediates the phosphoylation of downstream proteins which control hyphal branching in M. purpureus. The phosphoproteomes of the wild-type (WT) and ΔmpSTE1 strains were compared to pinpoint proteins specifically phosphorylated in an MpSTE1-dependent manner. Since the gene mpSTE1 was disrupted in the mutant, its targets should not undergo phosphorylation compared to the WT strain. The data were filtered for phosphorylated peptides present in all WT replicates but absent in all ΔmpSTE1 replicates.

ORGANISM(S): Monascus Purpureus

SUBMITTER: Mu Li

PROVIDER: PXD048208 | iProX | Fri Dec 29 00:00:00 GMT 2023

REPOSITORIES: iProX

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Publications

The serine/threonine protein kinase MpSTE1 directly governs hyphal branching in Monascus spp.

Duan Yali Y Chen Xizhu X Wang Tingya T Li Mu M

Applied microbiology and biotechnology 20240306 1

Monascus spp. are commercially important fungi due to their ability to produce beneficial secondary metabolites such as the cholesterol-lowering agent lovastatin and natural food colorants azaphilone pigments. Although hyphal branching intensively influenced the production of these secondary metabolites, the pivotal regulators of hyphal development in Monascus spp. remain unclear. To identify these important regulators, we developed an artificial intelligence (AI)-assisted image analysis tool fo ...[more]

PMID: 38446219

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Project description:The small G-protein KRAS is crucial for mediating gonadotropin-induced events associated with ovulation. However, constitutive expression of KrasG12D in granulosa cells disrupted normal follicle development leading to the persistence of abnormal follicle-like structures containing non-mitotic cells. To determine what factors mediate this potent effect of KrasG12D, gene profiling analyses were done. We also analyzed KrasG12D;Cyp19-Cre and KrasG12D;Pgr-Cre mutant mouse models that express Cre prior to or after the initiation of granulosa cell differentiation, respectively. KrasG12D induced cell cycle arrest in granulosa cells of the KrasG12D;Cyp19-Cre mice but not in the KrasG12D;Pgr-Cre mice, documenting the cell context specific effect of KrasG12D. Expression of KrasG12D silenced the Kras gene, reduced cell cycle activator genes and impaired expression of granulosa cell and oocyte specific genes. Conversely, levels of PTEN and phosphorylated p38MAPK increased markedly in the mutant granulosa cells. Because disrupting Pten in granulosa cells leads to increased proliferation and survival, Pten was disrupted in the KrasG12D mutant mice. The Pten/Kras mutant mice were infertile but lacked GCTs. By contrast, the Ptenfl/fl;KrasG12D;Amhr2-Cre mice developed aggressive ovarian surface epithelial (OSE) cell tumors that did not occur in the Ptenfl/fl;KrasG12D;Cyp19-Cre or Ptenfl/fl;KrasG12D;Pgr-Cre mouse strains. These data document unequivocally that Amhr2-Cre is expressed in and mediates allelic recombination of oncogenic genes in OSE cells. That KrasG12D/Pten mutant granulosa cells do not transform but rather undergo cell cycle arrest indicates that they resist the oncogenic insults of Kras/Pten by robust self-protecting mechanisms that silence the Kras gene and elevate PTEN and phospho-p38MAPK. Experiment Overall Design: Whole ovaries were collected from 26-day-old wild type mice, 26-day-old K-ras conditional mutant mice and 3-month-old K-ras conditional mutant mice. The gene expression profiles of these samples were compared using microarray method.

Dataset Information

Phosphoproteome of Monascus purpureus

Publications

The serine/threonine protein kinase MpSTE1 directly governs hyphal branching in Monascus spp.

Similar Datasets

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