Dataset Information

The Gland Secretions from Different Bufo species

ABSTRACT: Proteomic analysis of the gland secretions from different bufo species

ORGANISM(S): Bufo Gargarizans Gargarizans

SUBMITTER: Huimin Gao

PROVIDER: PXD048668 | iProX | Thu Jan 18 00:00:00 GMT 2024

REPOSITORIES: iProX

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Publications

Comprehensive Analysis of Bufadienolide and Protein Profiles of Gland Secretions from Medicinal Bufo Species.

Fang Yunge Y Chen Liangmian L Wang Pengfei P Liu Yating Y Wang Yuxiu Y Wang Zhimin Z Ma Yue Y Gao Huimin H

Toxins 20240320 3

Toad Venom (TV) is the dried product of toxic secretions from Bufo bufo gargarizans Cantor (BgC) or B. melanostictus Schneider (BmS). Given the increasing medical demand and the severe depletion of wild toads, a number of counterfeit TVs appeared on the market, posing challenges to its quality control. In order to develop an efficient, feasible, and comprehensive approach to evaluate TV quality, a thorough analysis and comparison of chemical compounds among legal species BgC and Bm ...[more]

PMID: 38535825

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Project description:Background: Anopheles culicifacies is a rural vector of malaria in tropical and sub tropical South East Asian region. The salivary gland of the mosquito is the target for sporozoite interaction, blood feeding behavior, haemostasis and vector-parasite interactions. Malaria parasite matures inside the salivary gland, gain competence and transmitted to the host along with the saliva during biting. The importance of the proteins expressed in salivary gland is the first step in understanding the physiology of blood feeding and may provide insights into vector- parasite interactions. Since, no genomic or transcriptomics information is available of Anopheles culicifacies, therefore locally expressed functional proteins in salivary glands are of much importance. . Method: In this study, 1DE protein and in solution digestion was combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for data mining was employed to study the proteome profile of salivary glands of sugar fed An. culicifacies mosquito species. Functional annotation of all the identified proteins was carried out using gene ontology tools, CELLO and SMART analysis software. Results: Total 102 proteins were identified and analysed by SEQUEST algorithm against mosquito protein database from Uniprot/NCBI. Out of which 81 proteins were identified using gel free approach and 21 proteins using in-gel approach and 15 were common among these two approaches. All the identified proteins were categorized in to 23 groups of biological processes using GO tool. 7 proteins were depicted to be secretary in nature by investigating the signal peptide present. Potential proteins with unknown function were predicted by analyzing their functional association with other characterized proteins by STRING algorithm and were categorized in cell adhesion, cytoskeleton and membrane trafficking networks. Conclusion: Our study elucidates the first proteomic dataset of An. culicifacies salivary gland proteins. Functional annotation of salivary proteins and complementary gene ontology assignments in An. culicifacies species may contribute towards understanding the complex physiology of the tissues in this species. This proteome baseline data may facilitate the discernment of salivary glands and parasite correlation during blood feeding. Furthermore, this mass spectrometry based proteomic data may also provide insights into the elucidation of role of differential functional proteins present in refractory An. culicifacies mosquito and may be useful for development of effective malaria control strategies.

Project description:The implanting embryo and developing placenta interface with the endometrium through a multinuclear trophoblast layer, syncytiotrophoblast (STB), which plays critical barrier, transport, immunomodulatory and endocrine roles throughout pregnancy. Understanding trophoblast and endometrial biology has recently been accelerated through sophisticated stem cell and organoid models, however few studies have combined these models to explore maternal-embryo communication. Here, we combined organoid models of secretory phase and decidual endometrial glands with trophoblast stem cell-derived STB to investigate signalling from maternal glands to the developing placenta. Luminal secretions collected from endometrial organoids by centrifugation were added to STB cultures and transcriptome analysis revealed that both secretory phase and decidual organoid secretions had profound effects on STB gene expression, inducing 3279 and 2634 differentially expressed genes (DEG), respectively (padj<0.05, >50% ≥1 log2 fold change). Gene set enrichment analysis of shared DEG (1926 genes) suggested upregulation of immune signalling and downregulation of mitochondrial respiration. Secretory and decidual secretions led to increased basal and maximal respiration (p<0.01) but reduced respiratory spare capacity (p<0.05) in STB, while glycolytic activity was also increased (p<0.05) together with increased expression of rate-limiting glycolysis enzymes HK1, HK2, PFKP and PKM (padj<0.03). Integrating cytokine array analysis of organoid secretions with upstream regulator analysis of STB DEG identified VEGFA and CSF1 as secretory products that might regulate mitochondrial and glycolytic genes, respectively, whereas secreted LCN2 and IL1A were upstream of immunomodulatory genes. Regulation of both immunomodulatory and metabolic gene sets was predicted for secreted CSF1 and EGF. The findings suggest that STB is highly responsive to endometrial gland secretions, which induce metabolic reprogramming likely to result in a more glycolytic phenotype in vivo as well as stimulating STB to modulate maternal immune responses. Endometrial gland signals thus shape trophoblast development to support successful establishment of pregnancy.

Dataset Information

The Gland Secretions from Different Bufo species

Publications

Comprehensive Analysis of Bufadienolide and Protein Profiles of Gland Secretions from Medicinal <i>Bufo</i> Species.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

The Gland Secretions from Different Bufo species

Publications

Comprehensive Analysis of Bufadienolide and Protein Profiles of Gland Secretions from Medicinal &lt;i&gt;Bufo&lt;/i&gt; Species.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Comprehensive Analysis of Bufadienolide and Protein Profiles of Gland Secretions from Medicinal <i>Bufo</i> Species.