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Functional study of EYA1 in glioma formation and progression

ABSTRACT: Phosphatase EYA1 was overexpressed in glioma cell line U87MG and phospho-proteomic analysis was performed to identify novel EYA1 substrates in glioma cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jian Zhang

PROVIDER: PXD048956 | iProX | Sun Jan 28 00:00:00 GMT 2024

REPOSITORIES: iProX

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Glioma-derived IL-33 orchestrates an inflammatory brain tumor microenvironment that accelerates glioma progression

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2011-04-16 | E-GEOD-28618 | biostudies-arrayexpress

Project description:cell culture:The human glioma cell line U87MG was obtained from the Cell Resource Center, Peking Union Medical College (Beijing, China), and U251MG was acquired from the American Type Culture Collection (Manassas, VA). Temozolomide (TMZ) resistant U87MG cells (U87TR) and TMZ resistant U251MG cells (U251TR) of glioblastoma (GBM) sub-cell lines, were established through repetitive exposure to increasing TMZ concentrations in vitro in our laboratory. Cells were cultured in DMEM culture medium supplemented with 10% FBS with a standard humidified incubator under 5% CO2 at 37°C.

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