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Lactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion

ABSTRACT: Embryonic stem cells (ESCs) were labeled with heavy isotopes, 13C615N4-arginine (Arg10) and 13C614N2-lysine (Lys6), or light isotopes, 12C614N4-arginine (Arg0) and 12C614N2-lysine (Lys0), respectively, for a total of 7 passages to ensure efficient labeling (>97%). The ESCs labeled with heavy isotopes were then treated with 20 mM SO for 24 hours before harvesting cells. Protein lysates of control and SO treated ESCs were prepared and mixed equivalently. Subsequently, trypsin digestion, high-performance liquid chromatography (HPLC) fractionation, Kla peptide enrichment with immobilized anti-Kla antibody, and high-resolution liquid chromatography–tandem MS (LC-MS/MS) were implemented. The resulting MS/MS data were processed using Maxquant search engine (v.1.5.2.8).

ORGANISM(S): Mus Musculus

SUBMITTER: Lingyi Chen

PROVIDER: PXD050051 | iProX | Wed May 01 00:00:00 BST 2024

REPOSITORIES: iProX

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Lactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion.

Dong Qiman Q Yang Xiaoqiong X Wang Lingling L Zhang Qingye Q Zhao Nannan N Nai Shanshan S Du Xiaoling X Chen Lingyi L

Stem cell research & therapy 20241113 1

<h4>Background</h4>Cellular metabolism regulates the pluripotency of embryonic stem cells (ESCs). Yet, how metabolism regulates the transition among different pluripotent states remains elusive. It has been shown that protein lactylation, which uses lactate, a metabolic product of glycolysis, as a substrate, plays a critical role in various biological events. Here we focused on that glycolysis regulates the conversion between ESCs and 2-cell-like cells (2CLCs) through protein lactylation.<h4>Met ...[more]

PMID: 39533309

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