Proteomics

Dataset Information

0

Mass spec results of Tmc7-GST pull-down

ABSTRACT: For the GST pull-down of DRG ganglion, the GST- or Tmc7-GST-associated beads were further incubated with cell lysates derived from DRG ganglion. The pulled-down proteins were then separated by SDS-PAGE gel electrophoresis and subsequently stained with silver staining Kit. Finally, the target molecular weight bands and undenatured beads were analyzed by proteomic analysis, respectively.

ORGANISM(S): Mus Musculus

SUBMITTER: Xiaona Du  

PROVIDER: PXD050466 | iProX | Fri Mar 08 00:00:00 GMT 2024

REPOSITORIES: iProX

Json Xml
altmetric image

Publications

TMC7 functions as a suppressor of Piezo2 in primary sensory neurons blunting peripheral mechanotransduction.

Zhang Xiaoxue X   Shao Jichen J   Wang Caixue C   Liu Chao C   Hao Han H   Li Xinmeng X   An Yating Y   He Jinsha J   Zhao Weixin W   Zhao Yiwen Y   Kong Youzhen Y   Jia Zhanfeng Z   Wan Shaopo S   Yuan Yi Y   Zhang Huiran H   Zhang Hailin H   Du Xiaona X  

Cell reports 20240402 4

The transmembrane channel-like (TMC) protein family comprises eight members, with TMC1 and TMC2 being extensively studied. This study demonstrates substantial co-expression of TMC7 with the mechanosensitive channel Piezo2 in somatosensory neurons. Genetic deletion of TMC7 in primary sensory ganglia neurons in vivo enhances sensitivity in both physiological and pathological mechanosensory transduction. This deletion leads to an increase in proportion of rapidly adapting (RA) currents conducted by  ...[more]

Similar Datasets

Transcriptomics [E-MTAB-308] Zebrafish gene 3 prime end pull down for genome annotation
2010-12-04 | GSE25023 | GEO
Proteomics βC1 proteins that were identified in GST-pull down coupled with mass spectrometry
2024-10-22 | PXD056381 | Pride
Proteomics Pseudomonas putida SQ1 pull-down
2024-07-10 | PXD047091 | Pride
Genomics RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
| PRJNA561512 | ENA
Proteomics Alkyne-probe pull-down in LAPC4-CR cells
2023-11-30 | MSV000093542 | MassIVE
Proteomics Proteomic profiling of proteins bound to lncRNA Cpat using RNA pull-down and mass spectrometry
2025-08-31 | MSV000098987 | MassIVE
Proteomics Identification of novel KRT32-associated proteins in primary keratinocytes cells lysate by GST-KRT32 pulldown assays coupled with mass spectrometry.
2024-06-17 | PXD050141 | Pride
Proteomics FILIP1 CT pull-down
2022-06-06 | PXD008875 | Pride
Proteomics Pull-down of Mlh3-Flag from yeast meiotic cells and analysis of pulled-down partner proteins
2020-11-06 | PXD014180 | Pride
Transcriptomics RNA pull-down analysis of hsa_circ_0001400 in 293T cells
2022-12-19 | GSE206929 | GEO