Dataset Information

Global profiling of protein lactylation in Human hippocampi

ABSTRACT: Lysine lactylation (Kla) is a novel posttranslational modification, playing important roles in numerous biological processes, including transcription, metabolism, tumorigenesis and nervous system diseases. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), researchers have identified lactylation in many human organs and other species, but no research on lactylation in human hippocampi has been reported. In this study, we performed global profiling of lactylation in human hippocampi under normal physiological conditions, and we identified 2579 Class I (localization probabilities > 0.75) lactylated sites in 853 proteins. Bioinformatics analysis showed that lactylated proteins were mainly located in the cytoplasm and involved in metabolic pathways and some neurological diseases. Compared with those reported lactylation databases, 434 lactylated proteins were identified for the first time in this study. Our work expands the database on human lactylation and helps advance the study on lactylation function and regulation under physiological and pathological conditions.

ORGANISM(S): Homo Sapiens

SUBMITTER: Juntao Yang

PROVIDER: PXD050470 | iProX | Fri Mar 08 00:00:00 GMT 2024

REPOSITORIES: iProX

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Publications

Global Profiling of Protein Lactylation in Human Hippocampi.

Shi Chun-Mei CM Wang Qiao-Chu QC Li Xiao-Lu XL Yang Ye-Hong YH Tang Xiao-Yue XY Wu Yue Y Ding Tao T Zhang Xu-Tong XT Zhang Zhi-Yi ZY Han Ron R Kong Jie J Liu Jiang-Feng JF Yang Jun-Tao JT

Proteomics. Clinical applications 20241128 2

<h4>Purpose</h4>The hippocampus has long been associated with cognition and memory function, the implications of lysine lactylation (Kla), a recently identified post-translational modification (PTM), in the role of the hippocampus remain largely unexplored.<h4>Experimental design</h4>An LC-MS/MS bottom-up proteomics analysis of three human hippocampal tissue samples was applied to profile the lactylation map in human hippocampi under normal physiological conditions.<h4>Results</h4>We identified ...[more]

PMID: 39610256

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Project description:Lysine lactylation (Kla) is a newly discovered histone post-translational modification (PTM), playing important roles in regulating transcription in macrophages. Increasing evidence demonstrates that lysine lactylation plays an important regulatory role in metabolic processes in both bacterial and human cells. However, little is known about the extent and function of lysine lactylation in fungi, here investigated with the black yeast Phialophora verrucosaverrucose as a model organism. WeHere, we report the first proteomic survey of this modification in P. verrucosa. We performed a global lactylation analysis of P. verrucosa using high accuracy bottom-up nano-LC-MS/MS in combination wwith the enrichment of lactylated peptides from digested cell lysates and subsequent peptide identification. In total, 636 lactylation sites on 420 lactylated proteins were identified in this pathogen, of which contained in 26 types of modification motifs. Our results show that over 85% of lactylated proteins were distributed in cytoplasm, mitochondria, and nucleus. The identified proteins were found to be involved associated toin diverse biological processes and were significantly enriched in the melanin biosynthesis process. Most strikingly, Kla was found in 23 structural constituent proteins of ribosome, indicating an impact of Kla in protein synthesis . Moreover, 12 lactylated proteins participated in fungal pathogenicity, suggesting a potential role for Kla in this process. Protein interaction network analysis suggested that a mass of protein interactions are regulated by lactylation. Together, our findings reveal widespread roles for lysine lactylation in regulating metabolism and melanin biosynthesis in P. verrucosa. Our data provide a rich resource for functional analyses of lactylation and facilitate the dissection of metabolic networks in this pathogen. The combined data sets represent the first report of the lactylome of P. verrucosa and provide a good foundation for further explorations of Kla in clinical fungal pathogens.

Dataset Information

Global profiling of protein lactylation in Human hippocampi

Publications

Global Profiling of Protein Lactylation in Human Hippocampi.

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