Nuclear HMGB1 is critical for CD8 T cell IFN-g production and anti-tumor immunity
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ABSTRACT: HMGB1 has been extensively studied as a DAMP, with secreted cytokine function. However, its regulation on T cells, especially the function in the nucleus, has not been elucidated. Here, we extracted the total protein of CD8 T cells and enriched the proteins bound to HMGB1 through IP experiment. We found several proteins binding with HMGB1, which suggested that nuclear HMGB1 might regulate the function of CD8 T cells by interacting with these proteins. In our study, we focused on the binding of HMGB1 and Eomes, and found that HMGB1 supported the expression of IFN-g in CD8 T cells by directly regulating the activity of the transcription factor Eomes of IFN-g. In conclusion, we determined the role of nuclear HMGB1 in the differentiation of CD8 T cells and proved that it played an important role in the anti-tumor program of CD8 T cells.
ORGANISM(S): Mus Musculus
SUBMITTER: Kebing Chen
PROVIDER: PXD050639 | iProX | Fri Mar 15 00:00:00 GMT 2024
REPOSITORIES: iProX
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