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MCPIP2 competes with IGF2BP1 binding to common stem-loop structure in the 3’UTRs of pro-angiogenic genes for suppressing breast tumor angiogenesis-2

ABSTRACT: This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human breast tumor cell MDA-MB-468, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wenbao Lu

PROVIDER: PXD052920 | iProX | Wed Jun 05 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX

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The RNA-Binding Proteins MCPIP2 and IGF2BP1 Competitively Modulate Breast Tumor Angiogenesis by Antagonizing VEGFA mRNA Stability and Expression.

Lu Wenbao W Li Hongwei H Liu Xueting X Li Ailing A Xiu Ruijuan R

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20250501 10

Tumor angiogenesis is essential for further growth and metastasis of solid tumors. However, the mechanisms underlying angiogenesis-related gene expression have yet to be clarified. Here, we discovered RNA-binding proteins monocyte chemotactic protein-induced protein 2 (MCPIP2) and insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) function as a pair of antagonists that modulate breast tumor angiogenesis by competitively regulating mRNA stability of proangiogenic gene transcripts, incl ...[more]

PMID: 40354028

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