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MCPIP2 competes with IGF2BP1 binding to common stem-loop structure in the 3’UTRs of pro-angiogenic genes for suppressing breast tumor angiogenesis-2

ABSTRACT: This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human breast tumor cell MDA-MB-468, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wenbao Lu

PROVIDER: PXD052920 | iProX | Wed Jun 05 00:00:00 BST 2024

REPOSITORIES: iProX

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DDX5 competes with ZC3H12D regulating CCND1 transcript stability to modulate cell cycle progression in breast cancer

Project description:This study was performed according to protein pull-down assay using GFP-coated beads in MDA-MB-468 cells expressing GFP/ZC3H12D fusion protein, and then the precipitated proteins were isolated by running SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human breast tumor cell MDA-MB-468, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.

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MCPIP2 competes with IGF2BP1 binding to common stem-loop structure in the 3’UTRs of pro-angiogenic genes for suppressing breast tumor angiogenesis

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Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch

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Anti-SLBP RIPchip data

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Transcription elongation rate affects nascent histone pre-mRNA folding and 3’ end processing

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Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIRP-Seq)

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Transcriptome-wide RNA structure probing reveals the structural basis of Dicer binding and cleavage

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SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

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Anti-SLBP RIPchip data

Project description:The animal replication-dependent (RD) histone mRNAs are coordinately regulated with chromosome replication. They are the only known cellular mRNAs that are not polyadenylated. Instead, the mature transcripts end in at a conserved stem-loop (SL) structure. This SL structure interacts stably with the stem-loop binding protein (SLBP), which is involved in all aspects of RD-histone mRNA metabolism. We used several genomic methods, including RNA Binding Protein IP followed by microarray analysis (RIP-ChIP) to analyze the RNA binding landscape of SLBP. Reference based design on two color Agilent DNA microarrays. Experimental sample is labeled in Ch1 (Cy3), which are mRNA bound by the SLBP RNA-binding protein. The reference sample is labeled in Ch2 (Cy5) which are the polyribosomal mRNAs from the supernatant that were not bound by anti-SLBP. Controls included no antibody in the IP (which tends to have non-specific background binding to the beads) and pre-incubation of the SLBP with the antibodies antigenic peptide, which blocks antibody binding to the SLBP.

2015-12-16 | E-GEOD-59915 | biostudies-arrayexpress

Pro-Calcific Environment Impairs Ischaemia-Driven Angiogenesis.

Project description:Peripheral arterial disease (PAD) is characterised by accelerated arterial calcification and impairment in angiogenesis. Studies implicate vascular calcification as a contributor to PAD, but the mechanisms remain unclear. We aimed to determine the effect of calcification on ischaemia-driven angiogenesis. Human coronary artery endothelial cells (ECs) were treated with calcification medium (CM: CaCl2 2.7 mM, Na2PO4 2.0 mM) for 24 h and exposed to normoxia (5% CO2) or hypoxia (1.2% O2; 5% CO2 balanced with N2). In normoxia, CM significantly inhibited tubule formation and migration and upregulated calcification markers of ALP, BMP2, and Runx2. CM elevated levels of calcification-protective gene OPG, demonstrating a compensatory mechanism by ECs. CM failed to induce pro-angiogenic regulators VEGFA and HIF-1α in hypoxia and further suppressed the phosphorylation of endothelial nitric oxide synthase (eNOS) that is essential for vascular function. In vivo, osteoprotegerin-deficient mice (OPG-/-), a calcification model, were subjected to hind-limb ischaemia (HLI) surgery. OPG-/- mice displayed elevated serum alkaline phosphatase (ALP) activity compared to wild-type controls. OPG-/- mice experienced striking reductions in blood-flow reperfusion in both 8-week-old and 6-month-old mice post-HLI. This coincided with significant impairment in tissue ischaemia and reduced limb function as assessed by clinical scoring (Tarlov). This study demonstrated for the first time that a pro-calcific environment is detrimental to ischaemia-driven angiogenesis. The degree of calcification in patients with PAD can often be a limiting factor with the use of standard therapies. These highly novel findings require further studies for full elucidation of the mechanisms involved and have implications for the development of therapies to suppress calcification in PAD.

| S-EPMC8954938 | biostudies-literature

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