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Low-input redoxomics facilitates global identification of metabolic regulators of oxidative stress in the gut

ABSTRACT: we developed a low-input and user-friendly pipeline for the simultaneous profiling of five distinct Cys states with regional resolution in the gut of monkeys, including free SH, total Cys oxidation (Sto), sulfenic acid (SOH), S-nitrosylation (SNO), and S-glutathionylation (SSG), shedding light on redox modification stoichiometries and redox signaling of gut aging.Furthermore, redox-modification and proteomic analysis of colonic tissues of calorie-restricted aged mice revealed that CR can reduce oxidative stress and highlight the protective effect of CR on intestinal health.

ORGANISM(S): Macaca Fascicularis Fascicularis Homo Sapiens Mus Musculus

SUBMITTER: Lunzhi Dai

PROVIDER: PXD053818 | iProX | Mon Dec 16 00:00:00 GMT 2024

REPOSITORIES: iProX

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Low-input redoxomics facilitates global identification of metabolic regulators of oxidative stress in the gut.

Xiao Xina X Hu Meng M Gao Li L Yuan Huan H Chong Baochen B Liu Yu Y Zhang Rou R Gong Yanqiu Y Du Dan D Zhang Yong Y Yang Hao H Liu Xiaohui X Zhang Yan Y Zhang Huiyuan H Xu Heng H Zhao Yi Y Meng Wenbo W Xie Dan D Lei Peng P Qi Shiqian S Peng Yong Y Tan Tao T Yu Yang Y Hu Hongbo H Dong Biao B Dai Lunzhi L

Signal transduction and targeted therapy 20250108 1

Oxidative stress plays a crucial role in organ aging and related diseases, yet the endogenous regulators involved remain largely unknown. This work highlights the importance of metabolic homeostasis in protecting against oxidative stress in the large intestine. By developing a low-input and user-friendly pipeline for the simultaneous profiling of five distinct cysteine (Cys) states, including free SH, total Cys oxidation (Sto), sulfenic acid (SOH), S-nitrosylation (SNO), and S-glutathionylation ...[more]

PMID: 39774148

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