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Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics

ABSTRACT: We present a high-sensitivity SCP workflow named Chip-Tip, identifying >5000 proteins in individual HeLa cells

ORGANISM(S): Homo Sapiens

SUBMITTER: Zilu Ye

PROVIDER: PXD054944 | iProX | Thu Aug 15 00:00:00 BST 2024

REPOSITORIES: iProX

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Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics.

Ye Zilu Z Sabatier Pierre P van der Hoeven Leander L Lechner Maico Y MY Phlairaharn Teeradon T Guzman Ulises H UH Liu Zhen Z Huang Haoran H Huang Min M Li Xiangjun X Hartlmayr David D Izaguirre Fabiana F Seth Anjali A Joshi Hiren J HJ Rodin Sergey S Grinnemo Karl-Henrik KH Hørning Ole B OB Bekker-Jensen Dorte B DB Bache Nicolai N Olsen Jesper V JV

Nature methods 20250116 3

Single-cell proteomics (SCP) promises to revolutionize biomedicine by providing an unparalleled view of the proteome in individual cells. Here, we present a high-sensitivity SCP workflow named Chip-Tip, identifying >5,000 proteins in individual HeLa cells. It also facilitated direct detection of post-translational modifications in single cells, making the need for specific post-translational modification-enrichment unnecessary. Our study demonstrates the feasibility of processing up to 120 label ...[more]

PMID: 39820750

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Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics.

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