Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics
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ABSTRACT:
ORGANISM(S): Homo Sapiens
SUBMITTER: 
Zilu Ye
PROVIDER: PXD054944 | iProX | Thu Aug 15 00:00:00 BST 2024
REPOSITORIES: iProX
Publications
Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics.
Ye Zilu Z Sabatier Pierre P van der Hoeven Leander L Lechner Maico Y MY Phlairaharn Teeradon T Guzman Ulises H UH Liu Zhen Z Huang Haoran H Huang Min M Li Xiangjun X Hartlmayr David D Izaguirre Fabiana F Seth Anjali A Joshi Hiren J HJ Rodin Sergey S Grinnemo Karl-Henrik KH Hørning Ole B OB Bekker-Jensen Dorte B DB Bache Nicolai N Olsen Jesper V JV
Nature methods 20250116 3
Single-cell proteomics (SCP) promises to revolutionize biomedicine by providing an unparalleled view of the proteome in individual cells. Here, we present a high-sensitivity SCP workflow named Chip-Tip, identifying >5,000 proteins in individual HeLa cells. It also facilitated direct detection of post-translational modifications in single cells, making the need for specific post-translational modification-enrichment unnecessary. Our study demonstrates the feasibility of processing up to 120 label ...[more]
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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
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