Dataset Information

Proteomics for CCL4-induced liver fibrosis

ABSTRACT: Proteomics for CCL4-induced liver fibrosis。 To understand the transcriptome data of liver fibrosis induced by CCl4 in mice. Methods: C57BL/6 mice were used to duplicate the liver fibrosis model induced by CCl-4. After successful replication, liver proteins were extracted from mice for subsequent experiments. Our experiment finally obtained protein data of CCL4-induced liver fibrosis model in mice, which provided further biological basis for our future study of liver fibrosis in mice.

ORGANISM(S): Mus Musculus

SUBMITTER: Tao Huang

PROVIDER: PXD055771 | iProX | Wed Sep 11 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Focal adhesion kinase promotes aerobic glycolysis in hepatic stellate cells via the cyclin D1/c-Myc/MCT-1 pathway to induce liver fibrosis.

Huang Tao T Zhou Ming-Yu MY Zou Gao-Liang GL Hu Rui-Han RH Han Lu L Zhang Qing-Xiu QX Zhao Xue-Ke XK

Scientific reports 20250207 1

Hepatic stellate cells (HSCs) transdifferentiate into myofibroblasts during liver fibrosis and exhibit increased glycolysis. Phosphorylated focal adhesion kinase (FAK) (pY397-FAK) promotes monocarboxylate transporter 1 (MCT-1) expression in HSCs to increase aerobic glycolysis and cause liver fibrosis. A combined multiomics analysis of C57BL/6 mice with tetrachloromethane (CCl<sub>4</sub>)-induced liver fibrosis was performed to identify the downstream FAK signaling pathway. The effect of the FAK ...[more]

PMID: 39915293

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Project description:Objective: To understand the transcriptome data of liver fibrosis induced by CCl4 in mice.Methods: C57BL/6 mice were used to duplicate the liver fibrosis model induced by CCl-4.After successful replication, mouse RNA was extracted for subsequent experiments.The basic principle of RNA extraction is to prevent RNA degradation during extraction, maximize RNA extraction efficiency, and ensure the extraction of high-quality RNA with good integrity and purity from target samples.According to the species (such as animal sample or plant sample) and sample status (such as animal tissue or animal cell) of the sample, select the appropriate extraction reagent and corresponding extraction method.The detection of high-quality RNA in samples is the basis for the success of the whole project. In order to ensure the accuracy of sequencing data, we performed quality inspection on samples, and the library construction can only be performed after the detection results meet the requirements for sequencing library construction.Sample test results are shown in the quality inspection report.After the library construction samples passed the test, the mRNA of eukaryotes was enriched with magnetic beads with Oligo (dT).Subsequently fragmentation buffer was added to randomly interrupt the mRNA.First-strand cDNA was synthesized using mRNA as a template with a six-base random primer (random hexamers), followed by buffer, dNTPs, and DNA polymerase I to synthesize the second-strand cDNA, which was subsequently purified using AMPure XP beads.Purified double-stranded cDNA was then end-repaired, A-tailed and ligated with sequencing adapters, followed by fragment size selection with AMPure XP beads, and finally PCR enrichment to obtain the final cDNA library.After library construction, insert size and effective concentration of the library were tested to ensure library quality.After passing the library test, different libraries were pooling according to the amount of data under the target and sequenced on the machine.RESULTS: RNA-seq data of CCL4-induced liver fibrosis model in mice were finally obtained in our experiment, which provided a molecular biological basis for our future study of liver fibrosis in mice.

Dataset Information

Proteomics for CCL4-induced liver fibrosis

Publications

Focal adhesion kinase promotes aerobic glycolysis in hepatic stellate cells via the cyclin D1/c-Myc/MCT-1 pathway to induce liver fibrosis.

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