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Chemoproteomic profiling reveals the mechanism of bile acid tolerance in pathogens

ABSTRACT: As amphipathic molecules, BAs have strong antibacterial effects, preventing overgrowth of the gut microbiota and defending the invasion of pathogens. However, some disease-causing pathogens can survive from the BA stress and knowledge is limited about how they develop BA tolerance. In the current study, we applied a quantitative chemoproteomic strategy to profile BA-interacting proteins in bacteria, aiming to discover the sensing pathway of BAs. By using the clickable and photo-affinity analogue BA probes with quantitative mass spectrometry, we identified 588 CA-interacting proteins and 435 DCA-interacting proteins.

ORGANISM(S): Escherichia Coli

SUBMITTER: Chu Wang

PROVIDER: PXD056639 | iProX | Wed Oct 09 00:00:00 BST 2024

REPOSITORIES: iProX

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Chemoproteomic Profiling Reveals the Mechanism of Bile Acid Tolerance in Bacteria.

Liu Biwei B Zhuang Shentian S Tian Runze R Liu Yuan Y Wang Yanqi Y Lei Xiaoguang X Wang Chu C

ACS chemical biology 20220901 9

Bile acids (BAs) are a class of endogenous metabolites with important functions. As amphipathic molecules, BAs have strong antibacterial effects, preventing overgrowth of the gut microbiota and defending the invasion of pathogens. However, some disease-causing pathogens can survive the BA stress and knowledge is limited about how they develop BA tolerance. In this work, we applied a quantitative chemoproteomic strategy to profile BA-interacting proteins in bacteria, aiming to discover the sensin ...[more]

PMID: 36049085

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