Dataset Information

Multi-organ Proteomic and Phosphoproteomic Profiles of Mpox Virus Infection in SIVmac239-infected Rhesus Macaques

ABSTRACT: Macaque study was conducted in an ABSL-3 facility, adhering to protocols approved by the Institutional Animal Care and Use Committee (IACUC) under the guidelines of the Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (Approval Number XJ23004). All animals were single-housed and cared for in a facility fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care. Six rhesus macaques (3 males and 3 females), aged 6–8 years, were inoculated with SIVmac239 at a dose of 100 TCID50 via the intravenous (I.V.) route. On day 115 post-challenge, these 6 rhesus macaques, along with 6 new rhesus macaques (3 males and 3 females, aged 6–8 years), were challenged with 1×107 TCID50 of MPXV through the I.V. route. Half of the rhesus macaques were necropsied on day 10 post-MPXV infection for tissue viral load assessment, histopathology, and proteomic and phosphoproteomic analyses.

ORGANISM(S): Macaca Mulatta

SUBMITTER: Juntao Yang

PROVIDER: PXD056753 | iProX | Sat Oct 12 00:00:00 BST 2024

REPOSITORIES: iProX

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Project description:Background Appropriate social interactions influence animal fitness by impacting several processes, such as mating, territory defense, and offspring care. Many studies shedding light on the neurobiological underpinnings of social behavior have focused on nonapeptides (vasopressin, oxytocin, and homologues) and on sexual or parent-offspring interactions. Furthermore, animals have been studied under artificial laboratory conditions, where the consequences of behavioral responses may not be as critical as when expressed under natural environments, therefore obscuring certain physiological responses. We used automated recording of social interactions of wild house mice outside of the breeding season to detect individuals at both tails of a distribution of egocentric network sizes (characterized by number of different partners encountered per day). We then used RNA-seq to perform an unbiased assessment of neural differences in gene expression in the prefrontal cortex, the hippocampus and the hypothalamus between these mice with naturally occurring extreme differences in social network size. Results We found that the neurogenomic pathways associated with having extreme social network sizes differed between the sexes. In females, hundreds of genes were differentially expressed between animals with small and large social network sizes, whereas in males very few were. In males, X-chromosome inactivation pathways in the prefrontal cortex were the ones that better differentiated animals with small from those with large social network sizes animals. In females, animals with small network size showed up-regulation of dopaminergic production and transport pathways in the hypothalamus. Additionally, in females, extracellular matrix deposition on hippocampal neurons was higher in individuals with small relative to large social network size. Conclusions Studying neural substrates of natural variation in social behavior in traditional model organisms in their habitat can open new targets of research for understanding variation in social behavior in other taxa.

Dataset Information

Multi-organ Proteomic and Phosphoproteomic Profiles of Mpox Virus Infection in SIVmac239-infected Rhesus Macaques

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