Dataset Information

Rapid adaption but genetic diversity loss of a globally distributed diatom in the warmer ocean

ABSTRACT: Studies have demonstrated that marine phytoplankton can adapt to the warmer environment. However, the underlying mechanisms remain largely unknown. Here, we quantified the capacity of a globally distributed marine diatom Skeletonema dohrnii, for rapid evolution under the moderate (24 ℃) and severe (28 ℃) warming scenarios. Whole-genome re-sequencing analysis revealed that the evolutionary adaptation of S. dohrnii to moderate warming was slow (i.e., 700 generations), whereas it was rapid (i.e., 300 generations) under severe warming but suffered a substantial loss of genetic diversity within the population. Genes associated with energy production and lipid metabolism evolved rapidly, particularly under severe warming, suggesting their vital roles in thermal adaptation. Proteomic results also showed the enhanced expression of proteins involved in energy production and lipid metabolism, especially under severe warming. Furthermore, particulate organic carbon and nitrogen production was greatly enhanced in the moderate warming-selected population but increased insignificantly in the severe warming-selected population, indicating more rapid adaptation driven by severe warming. Our results provide molecular insights into the rapid but limited evolution of thermal adaptation in marine diatoms and highlight energy production and lipid metabolism as the most important adaptive strategy. Future warming will affect genetic diversity and population dynamics of diatoms in the ocean.

ORGANISM(S): Skeletonema Dohrnii

SUBMITTER: Dazhi Wang

PROVIDER: PXD057232 | iProX | Sat Oct 26 00:00:00 BST 2024

REPOSITORIES: iProX

ACCESS DATA

Publications

Rapid Adaption but Genetic Diversity Loss of a Globally Distributed Diatom in the Warmer Ocean.

Cheng Luman L Xu Xiuming X Wang Minghua M Wang Da-Zhi DZ

Global change biology 20241201 12

Studies have demonstrated that marine phytoplankton can adapt to the warmer environment. However, the underlying mechanisms remain largely unknown. Here, we quantified the capacity of a globally distributed marine diatom Skeletonema dohrnii, for rapid evolution under the moderate (24°C) and severe (28°C) warming scenarios. Whole-genome resequencing analysis revealed that the evolutionary adaptation of S. dohrnii to moderate warming was slow (i.e., 700 generations), whereas it was rapid (i.e., 30 ...[more]

PMID: 39629602

Similar Datasets

Project description:Rainbow trout (Oncorhynchus mykiss), an important economically fish with high food value in China, is very sensitive to hypoxia. The gills are the first tissue to be attacked after hypoxia stress. However, the regulatory mechanisms in gills of rainbow trout to moderate and severe hypoxia stress are unclear. In this study, we determined the changes of biochemical parameters and gene expression in gills of rainbow trout during different periods of hypoxia (4, 8, 12, 24 h, and 1 month) and reoxygenation for 24 h, and analyzed the tissue and transcriptomic changes under moderate hypoxia for 12 h (Tm12G) and severe hypoxia for 12 h (Ts12G) compared with control (CG). The results showed that gill lamellae were bent and ILCM was reduced in the Tm12G group, while this phenomenon was more pronounced in the Ts12G group. At one month of hypoxia, we found that the gill lamellae epithelium was detached. Based on biochemical parameters and transcriptomic analysis, we found that the antioxidant and immune responses were activated, and glucose metabolism, lipid metabolism, and amino acid metabolism were enhanced, however, vitamin metabolism was suppressed under moderate hypoxia stress. Antioxidant and immune responses were activated, and glucose metabolism, lipid metabolism, and amino acid metabolism were enhanced, and nucleotide metabolism also was activated under severe hypoxia stress. From the analysis of gene expression pattern, we found that the expression of hsp90a, tnf, caspase3, mgst1, fih1, hif1a, ddit4, and egln3 showed a tendency to increase and then decrease and recovered after reoxygenation under moderate and severe hypoxia stress. Except for mgst1, the expression of other genes was significantly higher than the control at 12 h of stress. Under hypoxia stress, antioxidant and metabolic responses were first elevated and then decreased with time, and metabolic and immune pathways were activated, which coordinated with each other in response to hypoxia stress. These results provide basic information for an in-depth investigation of the physiological and molecular mechanisms in gills of rainbow trout under hypoxia stress.

Project description:Drought is one of the most important environmental fluctuations affecting tree growth and survival. Therefore, understanding of physiological and transcriptomic responses of trees to this stress factor will make important contributions to forest health and productivity. Here, we report comparative physiological and microarray based transcriptome analysis between drought resistant (N.62.191) and drought-sensitive (N.03.368.A) black poplar genotypes under well-watered (WWP), moderate drought (MD), severe drought (SD) and post drought re-watering (PDR) conditions. In the study, sensitive genotype exhibited a drought escape strategy with lower leaf water potential, higher reactive oxygen production, complete leaf abscission and subsequent terminal shoot necrosis under drought stress. On the other hand, resistant genotype had a dehydration tolerance indicating highly delayed leaf abscission under drought and fast growing capacity during re-watering conditions. Gene ontology enrichment analysis attributed drought susceptibility of black poplar to significant up-regulation of genes functional in transcription regulation (AP2/ERF, NAC and WRKY), cell wall modification (Expansins), fatty acid metabolism (enoyl-ACP reductase, lipid transport protein particle), protein degradation (endopeptidases), ethylene synthesis (1-aminocyclopropane-1-carboxylate) and riboflavin synthesis (GTP cyclohydrolase II) under drought stress. Transcriptomic comparison indicated significant down-regulation of photosynthesis, electron transport and carbohydrate metabolism related genes under drought stress in sensitive genotype. Although, similar reduction in carbohydrate metabolism was also recorded for resistant genotype, genes related with photosynthesis and electron transport systems were not down regulated even under SD for this genotype. Resistant genotype specific up-regulation of small heat shock proteins (sHSP) and bark storage proteins revealed importance of protein protection and nitrogen remobilization under drought stress, respectively. This is the first study associating BSP production to delayed leaf abscission and drought tolerance in trees. For Microarray experiment total RNA was isolated from the leaves randomly selected from two balck poplar seedlings (two biological replicates) for resistant and sensitive genotypes at well watered period (WWP), moderate drought (MD), severe drought (SD) and post drought rewatering (PDR) periods. For each water availability regime total isolated RNA was loaded onto two Affymetrix poplar Gene Chips for each genotype. Totally 16 Affymetrix poplar GeneChips (2 genotypes × 4 water availability regimes × 2 biological replicates) were used for transcriptional analysis.

Dataset Information

Rapid adaption but genetic diversity loss of a globally distributed diatom in the warmer ocean

Publications

Rapid Adaption but Genetic Diversity Loss of a Globally Distributed Diatom in the Warmer Ocean.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets