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Comprehensive N-Glycomics Profiling of Urinary Extracellular Vesicles Identifies a Novel Biomarker Panel for Diagnosis of Bladder Cancer

ABSTRACT: Bladder cancer (BC) is the most common urologic malignancy, facing enormous diagnostic challenges.Urinary extracellular vesicles (EVs) is a particularly promising source for developing diagnostic markers for bladder cancer because of the direct contact between urine and bladder. We investigate biomarkers for bladder cancer diagnosis based on an N-glycomics study involving the clinical cohort of 333 individuals. Finally, eight N-glycans were screened out to construct a diagnostic model, able to well differentiate bladder cancer from healthy individuals.To further understand the functions and potential roles of urinary EVs, we conducted N-glycoproteomic analysis of urinary EVs from the BC, UB, UM, and HC groups to explore the nature of glycoproteins carrying cancer-associated N-glycan signatures in EVs.

ORGANISM(S): Homo Sapiens

SUBMITTER: Haojie Lu

PROVIDER: PXD057882 | iProX | Thu Nov 14 00:00:00 GMT 2024

REPOSITORIES: iProX

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Urinary extracellular vesicle N-glycomics identifies diagnostic glycosignatures for bladder cancer.

Li Yang Y Fu Bin B Wang Maoyu M Chen Weiyu W Fan Jiawei J Li Yueyue Y Liu Xuejiao X Wang Jun J Zhang Zhensheng Z Lu Haojie H Zhang Ying Y

Nature communications 20250307 1

Bladder cancer (BC) is the most common urologic malignancy, facing enormous diagnostic challenges. Urinary extracellular vesicles (EVs) are promising source for developing diagnostic markers for bladder cancer because of the direct contact between urine and bladder. This study pioneers urinary EV N-glycomics for bladder cancer diagnosis. We have generated a comprehensive N-glycome landscape of urinary EVs through high-throughput N-glycome analysis, identifying a total of 252 N-glycans from 333 i ...[more]

PMID: 40055327

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