ABSTRACT: We expanded the shell mass used for shell matrix protein extraction and identification to 300g and identified matrix protein fractions in Crassostrea shells based on Astral mass spectrometer.
Project description:We expanded the shell mass used for the extraction and identification of shell matrix proteins to 300g, three distinct strategies (LC-MS/MS, PF-LC-MS/MS, and Astral mass spectrometer) were used to obtain the most complete shell matrix protein library of Crassostrea gigas
Project description:Studying the adaptive divergence of shellfish inhabiting different environments is crucial to predict the resilience of marine organisms to rapid climate change. Although the shell serves as the primary physical barrier against environmental change, the evolutionary adaptation of biomineralization in shellfish remains poorly understood. In this study, we using common garden designs to investigate the shell matrix proteome of estuarine (Crassostrea ariakensis) and Pacific (Crassostrea gigas) oysters inhabiting estuarine and open coastal zones, respectively. Shell matrix proteome analyses revealed extensive domain expansion of classical pathway secretomes, which likely contribute to the enhanced biomineralization capacity of estuarine oysters. Furthermore, two-thirds of the 27 C. ariakensis-specific shell matrix-secreted proteins (SMSPs) lacked homology with known proteins in the Swiss-Prot and nr databases, indicating rapid evolution. Our findings suggest that intensified classical pathway secretomes and rapid evolution of species-specific SMSPs are key factors shaping the defense of shells to enhance their adaptive potential to climate change.
Project description:Deep sequencing of mRNA from Pacific oyster Crassostrea gigas Competent larvae of Crassostrea gigas were treated with epinephrine solution, and then sampled at different time intervals. For shell damage experiment, shell were broken and then tissues were sampled at different time intervals.
Project description:This project contains proteomic profiles of shell matrix proteins (SMPs) from the Iwagaki oyster Crassostrea nippona, covering both life stage-specific shells (D-stage larval and adult shell) and shell microstructure-specific layers (prismatic, foliated, and chalky layers of the adult shell). These datasets were generated to investigate the molecular composition and regulatory mechanisms underlying biomineralized shell formation across developmental stages and shell architectures. The identified proteins provide insights into shell mineralization from both structural and evolutionary perspectives.
Project description:Deep sequencing of samples from different development stages, different adult organs and different stress treatments of Pacific oyster Crassostrea gigas
Project description:Here we reported 226 sperm proteins from the Hong Kong oyster Crassostrea hongkongensis. Proteins extracted from three sperm samples were separated by SDS-PAGE, analyzed by LC-MS/MS and identified using Mascot.
Project description:Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O2 L-1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. This is the first study employing microarrays to characterize the genetic markers and metabolic pathways responding to hypoxic stress in C. gigas.