Dataset Information

Study on the spatial effect of peripheral ApoE3 on regulating cadmium binding proteome and mechanism of cognitive impairment in the elderly

ABSTRACT: The experiment utilized a total of 54 five-week-old Sprague-Dawley rats, each weighing approximately 100 grams. These rats were randomly divided into 9 groups, consisting of 27 female and 27 male rats. Based on the intervention dose of cadmium (Cd), the rats were further randomly assigned into 3 main groups, each containing 18 rats: the control group received 1 milliliter of sodium chloride solution daily; the low-dose group received 1 milliliter of cadmium chloride (CdCl₂) solution at a concentration of 30 micrograms per kilogram (μg/kg) daily; and the high-dose group received 1 milliliter of cadmium chloride (CdCl₂) solution at a concentration of 1 milligram per kilogram (mg/kg) daily. Cadmium exposure was administered via oral gavage. Each dose group (control group, low-dose group, and high-dose group) was intervened at three different time points: 2 weeks, 4 weeks, and 8 weeks. At each intervention time point, 6 rats were randomly selected from each dose group for euthanasia, and their prefrontal brain tissues were collected for subsequent analysis. During the experiment, some rats died: 2 rats in the control group died at 2 weeks, and 1 rat in the high-dose group died at 4 weeks and 8 weeks, respectively. The experimental design ensured a systematic evaluation of the effects of cadmium exposure on rats under different doses and time points, while recording the mortality during the experiment for further analysis.

ORGANISM(S): Rattus Rattus

SUBMITTER: Shaoxin Huang

PROVIDER: PXD063013 | iProX | Wed Apr 16 00:00:00 BST 2025

REPOSITORIES: iProX

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Publications

Proteomic signatures and predictive modeling of cadmium-associated anxiety in middle-aged and elderly populations: an environmental exposure association study.

Wan Sheng S Yang Yong Y Zhao Qihan Q Xing Zelong Z Li Jie J Gao Hao H Yin Yinghui Y Liu Zhenzhong Z Chen Qiwen Q Tian Maoqin M Shi Xinxin X Ji Ziyue Z Huang Shaoxin S

Journal of translational medicine 20250501 1

<h4>Background</h4>Emerging evidence implicates environmental contaminants such as cadmium (Cd) as modifiable risk factors for anxiety. Despite growing recognition of heavy metal toxicity in neuropsychiatric disorders, the molecular mechanisms linking environmental exposure to anxiety pathogenesis remain poorly understood.<h4>Methods</h4>Based on the established cohort of individuals with cognitive impairment in cadmium-contaminated areas, this cross-sectional association study enrolled 50 middl ...[more]

PMID: 40312359

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Project description:The prevalence of obesity has significantly increased worldwide. Therefore, this study aimed to evaluate the influence of obesity in the proteomic profile of periodontal ligament (PDL) tissues of rat first maxillary molars submitted to orthodontic tooth movement. The study was approved by the Ethical Committee on Animal Experimentation of the Sao Paulo State University, School of Dentistry at Araraquara, Brazil (protocol number 16/2015) and followed the Animal Research: Reporting of In Vivo Experiments guidelines. Therefore, for this study, we used ten male adult Holtzman rats (weight average – 300 g, approximately 8 weeks old). Animals were randomly assigned to one of the following experimental groups: 1 – M group: animals received a standard diet and 75 days after that, the first maxillary molar was randomly selected to be submitted to OTM for 15 days (n=5), and 2 – OM group: animals received a high-fat diet to induce obesity, and 75 days after that the first maxillary molar was randomly selected to be subjected to OTM for 15 days (n=5). All animals were kept under controlled temperature (22-25 °C), receiving water ad libitum with a 12-hour light/dark cycle. To induce obesity, animals of the OM group received a high-fat diet. In contrast to the standard diet (Labina/Purina, Ribeirão Preto, Brazil) administered to the M group. The high-fat diet contained around 3.82 kcal/g and was composed of 27.5 % of standard rat chow plus 25 % of peanuts, 25 % of milk chocolate, and 12.5 % sweet biscuits and contained 20 g of protein, 48 g of carbohydrate, 20 g of total fat, and 4 g of fiber per 100 g of diet. This feed was daily replaced and had started 75 days before baseline (M initiation). The procedure to induce OTM was by a closed coil nickel-titanium spring (Sentalloy, GAC Central Slip, New York, EUA) connected the maxillary first molar and central incisors with a steel wire (CrNi, 0.20 mm, 55.01.208; Morelli, Sorocaba, Brazi). Fifteen days after OTM, animals were euthanized by anesthetic overdose.

Dataset Information

Study on the spatial effect of peripheral ApoE3 on regulating cadmium binding proteome and mechanism of cognitive impairment in the elderly

Publications

Proteomic signatures and predictive modeling of cadmium-associated anxiety in middle-aged and elderly populations: an environmental exposure association study.

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